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Nucleostemin Antibody (2C8D5) - BSA Free

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Western Blot: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Western blot analysis using GNL3 mouse mAb against NIH3T3 (1) and PC-3 (2) cell lysate.
Flow Cytometry: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Flow cytometric analysis of Jurkat cells using GNL3 mouse mAb (green) and negative control (purple).
Western Blot: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Western blot analysis using GNL3 mAb against human GNL3 recombinant protein. (Expected MW is 51.9 kDa)
Western Blot: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Western blot analysis using GNL3 mAb against HEK293 (1) and GNL3 (AA: 1-226)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, CyTOF-ready
Clone
2C8D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Nucleostemin Antibody (2C8D5) - BSA Free Summary

Immunogen
Purified recombinant fragment of human Nucleostemin (AA: 1-226) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GNL3
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Nucleostemin Antibody (2C8D5) - BSA Free

  • C77032
  • E2IG3NNP47
  • estradiol-induced nucleotide binding protein
  • GNL3
  • guanine nucleotide binding protein-like 3 (nucleolar)
  • guanine nucleotide-binding protein-like 3
  • MGC800
  • Novel nucleolar protein 47
  • NSE2-induced gene 3 protein
  • Nucleolar GTP-binding protein 3
  • Nucleostemin

Background

Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GNL3