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NT5C Antibody

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Western Blot: NT5C Antibody [NBP1-84564] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Western Blot: NT5C Antibody [NBP1-84564] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: NT5C Antibody [NBP1-84564] - Staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
Western Blot: NT5C Antibody [NBP1-84564] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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NT5C Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GALDAVREMNDLPDTQVFICTSPLLKYHHCVGEKYRWVEQHLGPQFVERIILTRDKTVVL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NT5C
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
NT5C Protein (NBP1-84564PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NT5C Antibody

  • 3'-pyrimidine nucleotidase
  • 5' nucleotidase, deoxy (pyrimidine), cytosolic type C
  • 5', 3'-nucleotidase, cytosolic
  • cdN
  • Cytosolic 5'
  • Deoxy-5'-nucleotidase 1
  • dNT-15'(3')-deoxyribonucleotidase, cytosolic type
  • DNT1DNT-1
  • EC 3.1.3.-
  • P5N2
  • PN-I
  • PN-II
  • UMPH2DNT
  • uridine 5'-monophosphate phosphohydrolase 2
  • uridine 5-prime monophosphate hydrolase 2

Background

Pyrimidine 5-prime nucleotidase (P5N; EC 3.1.3.5), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1; MIM 606224) and type II (UMPH2).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NT5C Antibody
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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol NT5C