Home » NT5C2 » NT5C2 Antibodies » NT5C2 Antibody (3C1)
Novus Biologicals products are now on bio-techne.com

NT5C2 Antibody (3C1)

 (8 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: NT5C2 Antibody (3C1) [H00022978-M02] - Analysis of NT5C2 expression in transfected 293T cell line by NT5C2 monoclonal antibody (M02), clone 3C1.Lane 1: NT5C2 transfected lysate(64.97 KDa).Lane 2: Non-transfected lysate.
Western Blot: NT5C2 Antibody (3C1) [H00022978-M02] - Analysis of NT5C2 expression in transfected 293T cell line by NT5C2 monoclonal antibody (M02), clone 3C1.Lane 1: NT5C2 transfected lysate(64.97 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: NT5C2 Antibody (3C1) [H00022978-M02] - Analysis of monoclonal antibody to NT5C2 on HeLa cell . Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: NT5C2 Antibody (3C1) [H00022978-M02] - Analysis of monoclonal antibody to NT5C2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
3C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

NT5C2 Antibody (3C1) Summary

Immunogen
NT5C2 (AAH01595, 1 a.a. ~ 561 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSTSWSDRLQNAADMPANMDKHALKKYRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESPLATRNRTSVDFKDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEEEEE
Specificity
NT5C2 - 5'-nucleotidase, cytosolic II
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NT5C2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Publications
Read Publications using H00022978-M02.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NT5C2 Antibody (3C1)

  • 5'-nucleotidase (purine), cytosolic type B
  • 5'-nucleotidase, cytosolic II
  • cN-II
  • Cytosolic 5'-nucleotidase II
  • EC 3.1.3
  • EC 3.1.3.5
  • GMP
  • NT5BIMP-specific 5'-NT
  • NT5CP
  • PNT5cytosolic purine 5'-nucleotidase
  • purine 5' nucleotidase

Background

Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...


5'-Nucleotidase/CD73 A ...
NBP2-48480
Flow (Cell Surface) 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free
Flow (Cell Surface) 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free
Flow Cytometry 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free
(1 Review)
(15 Publications)
Species: Hu
Applications: Flow-CS, Flow, IHC, IHC-Fr, IHC-P
Prostatic Acid
Phosphatase/ACPP Anti ...
AF6240
Western Blot Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]
Immunohistochemistry Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]
ELISA Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]
(1 Publication)
Species: Hu
Applications: ELISA, IHC, WB
DCK Antibody
(10412)
NBP2-43639
Western Blot DCK Antibody (10412)
Immunocytochemistry/ Immunofluorescence DCK Antibody (10412)
Western Blot DCK Antibody (10412)
(1 Review)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
ENT1 Antibody
NBP1-84838
Immunohistochemistry-Paraffin ENT1 Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P
BRAP Antibody
NBP1-91705
Immunocytochemistry/ Immunofluorescence BRAP Antibody
Immunohistochemistry-Paraffin BRAP Antibody
Immunohistochemistry-Paraffin BRAP Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
IMPA1 Antibody
NBP1-83107
Western Blot IMPA1 Antibody
Immunocytochemistry/ Immunofluorescence IMPA1 Antibody
Immunohistochemistry-Paraffin IMPA1 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Citidine Deaminase
Antibody
NBP2-39019
Immunocytochemistry/ Immunofluorescence Citidine Deaminase Antibody
Immunohistochemistry-Paraffin Citidine Deaminase Antibody
Immunohistochemistry-Paraffin Citidine Deaminase Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NT5C3 Antibody
NBP1-85525
Western Blot NT5C3 Antibody
Immunohistochemistry-Paraffin NT5C3 Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
RRM2 Antibody
NBP1-31661
Western Blot RRM2 Antibody
Immunohistochemistry RRM2 Antibody
Immunohistochemistry-Paraffin RRM2 Antibody
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-P, WB
RRM1 Antibody
(HL1231) - Azide and ...
NBP3-25643
Western Blot RRM1 Antibody (HL1231) - Azide and BSA Free
Western Blot RRM1 Antibody (HL1231) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence RRM1 Antibody (HL1231) - Azide and BSA Free
Species: Ca, Fe, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
CD82/Kai-1
Antibody - BSA Free
NBP1-76775
Immunocytochemistry/ Immunofluorescence CD82/Kai-1 Antibody - BSA Free
Immunohistochemistry CD82/Kai-1 Antibody - BSA Free
Immunohistochemistry CD82/Kai-1 Antibody - BSA Free
(2 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB

Glucuronosyltransferas ...
NBP3-03338
Immunohistochemistry-Paraffin Glucuronosyltransferase 1A1/UGT1A1 Antibody - Azide and BSA Free
Immunohistochemistry Glucuronosyltransferase 1A1/UGT1A1 Antibody - Azide and BSA Free
Immunohistochemistry Glucuronosyltransferase 1A1/UGT1A1 Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MID1 Antibody -
BSA Free
NBP3-05061
Immunocytochemistry/ Immunofluorescence MID1 Antibody - BSA Free
Western Blot MID1 Antibody - BSA Free
Species: Hu, Mu
Applications: ICC/IF, WB
CD1e Antibody
(704407) [Unconjugate
MAB7330
Intracellular Staining by Flow Cytometry CD1e Antibody (704407) [Unconjugated]
Species: Hu
Applications: CyTOF-ready, ICFlow
CD1b Antibody
(737249) [Unconjugate
MAB7446
Flow Cytometry CD1b Antibody (737249) [Unconjugated]
(1 Publication)
Species: Hu
Applications: CyTOF-ready, Flow
CDCA7L Antibody
(OTI3B5)
NBP2-46198
Western Blot CDCA7L Antibody (OTI3B5)
Immunohistochemistry CDCA7L Antibody (OTI3B5)
Immunohistochemistry CDCA7L Antibody (OTI3B5)
(1 Review)
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
NT5C Antibody
NBP1-84563
Immunocytochemistry/ Immunofluorescence NT5C Antibody
Knockdown Validated NT5C Antibody
Western Blot NT5C Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, Simple Western, WB
Cytochrome P450
17A1 Antibody (OTI3F1
NBP2-01151
Immunohistochemistry Cytochrome P450 17A1 Antibody (OTI3F11)
Immunohistochemistry Cytochrome P450 17A1 Antibody (OTI3F11)
Flow Cytometry Cytochrome P450 17A1 Antibody (OTI3F11)
(2 Reviews)
(6 Publications)
Species: Hu
Applications: Flow, IHC, IHC-P, WB

Publications for NT5C2 Antibody (H00022978-M02)(8)


Showing Publications 1 - 8 of 8.
Publications using H00022978-M02 Applications Species
Muhammad-Zawwad R, Octavia C, Charles D et al. CD73 and cN-II regulate the cellular response to chemotherapeutic and hypoxic stress in lung adenocarcinoma cells. Biochim Biophys Acta Gen Subj. 2021-01-09 [PMID: 33434633]
Cadassou O, Raza MZ, Machon C et al. Enhanced migration of breast and lung cancer cells deficient for cN-II and CD73 via COX-2/PGE2/AKT axis regulation Cell Oncol (Dordr) 2020-09-24 [PMID: 32970317]
Duarte R, Bachtel N, Cotel M et al. The Psychiatric Risk Gene NT5C2 Regulates Adenosine Monophosphate-Activated Protein Kinase Signaling and Protein Translation in Human Neural Progenitor Cells. Biol Psychiatry. 2019-03-30 [PMID: 31097295]
Schneider C, Oellerich T, Baldauf HM et al. SAMHD1 is a biomarker for cytarabine response and a therapeutic target in acute myeloid leukemia. Nat Med 2016-12-19 [PMID: 27991919]
Cividini F, Cros-Perrial E, Pesi R et al. Cell proliferation and drug sensitivity of human glioblastoma cells are altered by the stable modulation of cytosolic 5'-nucleotidase II. Int J Biochem Cell Biol. 2015-06-14 [PMID: 26079827]
Jordheim LP, Puy JY, Cros-Perrial E et al. Determination of the enzymatic activity of cytosolic 5'-nucleotidase cN-II in cancer cells: development of a simple analytical method and related cell line models. Anal Bioanal Chem. 2015-05-22 [PMID: 25998135]
Cividini F, Tozzi MG, Galli A et al. Cytosolic 5-Nucleotidase II Interacts with the Leucin Rich Repeat of NLR Family Member Ipaf. PLoS One. 2015-03-26 [PMID: 25811392]
Kulkarni SS, Karlsson HK, Szekeres F et al. Suppression of 5'-Nucleotidase Enzymes Promotes AMP-activated Protein Kinase (AMPK) Phosphorylation and Metabolism in Human and Mouse Skeletal Muscle. J Biol Chem. 2011-08-26 [PMID: 21873433]

Reviews for NT5C2 Antibody (H00022978-M02) (0)

There are no reviews for NT5C2 Antibody (H00022978-M02). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NT5C2 Antibody (H00022978-M02) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional NT5C2 Products

Blogs on NT5C2

There are no specific blogs for NT5C2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NT5C2 Antibody (3C1) and receive a gift card or discount.


Bioinformatics

Gene Symbol NT5C2
Entrez
OMIM
Uniprot