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NSUN5 Antibody

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Western Blot: NSUN5 Antibody [NBP1-89416] - Analysis in human cell lines Caco-2 and U-251MG using anti-NSUN5 antibody. Corresponding NSUN5 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Orthogonal Strategies: Western Blot: NSUN5 Antibody [NBP1-89416] - Analysis in human cell lines Caco-2 and U-251MG using anti-NSUN5 antibody. Corresponding NSUN5 RNA-seq data are presented for the same cell ...read more
Immunocytochemistry/ Immunofluorescence: NSUN5 Antibody [NBP1-89416] - Staining of human cell line A-431 shows positivity in nucleus and nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NSUN5 Antibody [NBP1-89416] - Staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.
Western Blot: NSUN5 Antibody [NBP1-89416] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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NSUN5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QLPRFVRVNTLKTCSDDVVDYFKRQGFSYQGRASSLDDLRALKGKHFLLDPLMPELLVFPAQTDLHEHPLYRAGHLIL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NSUN5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NSUN5 Protein (NBP1-89416PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NSUN5 Antibody

  • EC 2.1.1.-
  • FLJ10267
  • member 5A
  • MGC986
  • NOL1
  • NOL1/NOP2/Sun domain family member 5
  • NOL1/NOP2/Sun domain family, member 5
  • NOL1R(NOL1)
  • NOL1-related protein
  • NOP2/Sun domain family, member 5
  • NSUN5A
  • p120
  • putative methyltransferase NSUN5
  • WBSCR20
  • WBSCR20A
  • Williams-Beuren syndrome chromosomal region 20A protein
  • Williams-Beuren syndrome critical region protein 20 copy A
  • Ynl022cL

Background

NSUN5 encodes a protein with similarity to p120 (NOL1), a 120-kDa proliferation-associated nucleolar antigen that is a member of an evolutionarily conserved protein family. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NSUN5