![Western Blot: Nrf1 Antibody [NBP1-77822] - Lane: NFR1-HIS recombinant protein. Load: 50ug per lane. Primary antibody - 1: NRF1 antibody at 1:1,000 overnight at 4C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-77822-img0003.jpg "Western Blot: Nrf1 Antibody [NBP1-77822] - Lane: NFR1-HIS recombinant protein. Load: 50ug per lane. Primary antibody - 1: NRF1 antibody at 1:1,000 overnight at 4C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This protein A purified antibody is directed against mouse Nrf1. The product was purified from monospecific antiserum by protein A affinity purification Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | This protein A purified Nrf1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant mouse Nrf1 protein corresponding to aa 1- 534 of the native protein. (Uniprot: Q9WU00) |
| Specificity | This protein A purified antibody is directed against mouse Nrf1. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NRF1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 67 kDa in size corresponding to NRF1 by western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands. |
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| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry: Nrf1 Antibody [NBP1-77822] - Used at a 5 ug/ml to detect NRF1 in a variety of tissues. This image shows NRF1 staining of human testis. The antibody shows nuclear staining in lymphocytes. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-NBP1-77822-img0004.jpg "Immunohistochemistry: Nrf1 Antibody [NBP1-77822] - Used at a 5 ug/ml to detect NRF1 in a variety of tissues. This image shows NRF1 staining of human testis. The antibody shows nuclear staining in lymphocytes. Tissue was formalin-fixed and paraffin embedded.")
![Western Blot: Nrf1 Antibody [NBP1-77822] - Shows detection of a 67-kDa band corresponding to human NRF1 in a (lane 1) HeLa nuclear extract and (lane 2) whole cell lysate (molecular weight marker not shown). Approx. 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye (TM) 700 labeled Goat-a-Rabbit IgG [H&L] diluted 1:2,500. IRDye (TM) 700](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-77822-img0002.jpg "Western Blot: Nrf1 Antibody [NBP1-77822] - Shows detection of a 67-kDa band corresponding to human NRF1 in a (lane 1) HeLa nuclear extract and (lane 2) whole cell lysate (molecular weight marker not shown). Approx. 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye (TM) 700 labeled Goat-a-Rabbit IgG [H&L] diluted 1:2,500. IRDye (TM) 700")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")