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NNT Antibody

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Western Blot: NNT Antibody [NBP1-32109] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Mouse kidney. NNT antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: NNT Antibody [NBP1-32109] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - SW480 xenograft, using NNT antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Mouse stomach. NNT antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunocytochemistry/ Immunofluorescence: NNT Antibody [NBP1-32109] - NNT antibody detects NNT protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: NNT ...read more
Genetic Strategies: Western Blot: NNT Antibody [NBP1-32109] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, XpSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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NNT Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human NNT. The exact sequence is proprietary.
Localization
Mitochondrion inner membrane, Multi-pass membrane protein, Matrix side
Predicted Species
Bovine (99%), Xenopus (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NNT
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (88%), Xenopus laevis (93%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for NNT Antibody

  • MGC126502
  • mitochondrial
  • nicotinamide nucleotide transhydrogenaseMGC126503
  • Pyridine nucleotide transhydrogenase

Background

This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants, encoding the same protein, have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NNT