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nNOS Antibody [Unconjugated]

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Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat nNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2416) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for nNOS at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat nNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2416) followed ...read more
nNOS was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 1.7 µg/mL Goat Anti-Human/Mouse/Rat nNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2416) overnight at 4 ...read more
nNOS was detected in immersion fixed frozen sections of rat brain (cortex) using Goat Anti-Human/Mouse/Rat nNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2416) at 5 µg/mL overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of mouse brain tissue and rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for nNOS at approximately 160 kDa (as indicated) using 10 µg/mL of Goat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2416-SP
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nNOS Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human nNOS
Ser218-Ser1434
Accession # P29475
Specificity
Detects human, mouse, and rat nNOS in Western blots. In direct ELISAs, this antibody shows approximately 5% cross‑reactivity with recombinant human eNOS.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NOS1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF2416 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for nNOS Antibody [Unconjugated]

  • bNOS
  • brain
  • Constitutive NOS
  • EC 1.14.13.39
  • IHPS1
  • Neuronal NOS
  • nitric oxide synthase 1 (neuronal)
  • nNOS
  • N-NOS
  • nNOSNOS type I
  • NOS
  • NOS1
  • Peptidyl-cysteine S-nitrosylase NOS1

Background

nNOS is one of three NOS enzymes that catalyze the oxidation of L-argine to L-citruline and nitric oxide. nNOS exists as homodimers containing a cytochrome P450‑like prosthetic heme group in the N-terminal half. It also has a tightly bound FAD and FMN group in the C-terminal half. At least 4 isoforms of human nNOS are known. Human nNOS shares about 55% amino acid sequence identity with eNOS and iNOS. It also shares 96% sequence identity with mouse or rat nNOS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for nNOS Antibody (AF2416)(3)

We have publications tested in 5 confirmed species: Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Saimiri sciureus (South American Squirrel Monkey).

We have publications tested in 2 applications: IHC, Simple Western.


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(1)
Simple Western
(1)
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(1)
Mouse
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Primate - Saimiri sciureus (South American Squirrel Monkey)
(1)
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Reviews for nNOS Antibody (AF2416) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for nNOS Antibody (AF2416) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NOS1
Uniprot