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NMT2 Antibody

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Genetic Strategies: Western Blot: NMT2 Antibody [NBP1-85817] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading ...read more
Immunocytochemistry/ Immunofluorescence: NMT2 Antibody [NBP1-85817] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NMT2 Antibody [NBP1-85817] - Staining of human Testis shows strong membranous and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: NMT2 Antibody [NBP1-85817] - Staining of human Cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: NMT2 Antibody [NBP1-85817] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: NMT2 Antibody [NBP1-85817] - Staining of human Tonsil shows moderate membranous positivity in non-germinal center cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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NMT2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GGTKSDSASDSQEIKIQQPSKNPSVPMQKLQDIQRAMELLSACQGPARNIDEAAKHRYQFWDTQPVPKLDEVITSHGAIEPDKDNVRQEPYSLPQGFMWDTLDLSDAEVLKELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGWLL
Predicted Species
Mouse (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NMT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NMT2 Protein (NBP1-85817PEP)
Publications
Read Publication using NBP1-85817.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NMT2 Antibody

  • EC 2.3.1.97
  • glycylpeptide N-tetradecanoyltransferase 2
  • Myristoyl-CoA:protein N-myristoyltransferase 2
  • NMT 2
  • N-myristoyltransferase 2
  • Peptide N-myristoyltransferase 2
  • Type II N-myristoyltransferase

Background

N-myristoyltransferase (NMT) catalyzes the reaction of N-terminal myristoylation of many signaling proteins. It transfers myristic acid from myristoyl coenzyme A to the amino group of a protein's N-terminal glycine residue. Biochemical evidence indicates the presence of several distinct NMTs, varying in apparent molecular weight and /or subcellular distribution. The predicted 498-amino acid of human NMT2 protein shares 77% and 96% sequence identity with human NMT1 and mouse Nmt2 comprise two distinct families of N-myristoyltransferases. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NMT2 Antibody (NBP1-85817)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NMT2 Antibody (NBP1-85817) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on NMT2

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Bioinformatics

Gene Symbol NMT2