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NME4 Antibody (OTI1A5)

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Western Blot: NME4 Antibody (1A5) [NBP2-02487] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NME4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: NME4 Antibody (1A5) [NBP2-02487] Staining of COS7 cells transiently transfected by pCMV6-ENTRY NME4.
Immunohistochemistry-Paraffin: NME4 Antibody (1A5) [NBP2-02487] - Staining of paraffin-embedded liver tissue using anti-NME4 mouse monoclonal antibody.
Flow Cytometry: NME4 Antibody (1A5) [NBP2-02487] - HEK293T cells transfected with either pCMV6-ENTRY NME4.(Red) or empty vector control plasmid (Blue) were immunostained with anti-NME4 mouse monoclonal, and then ...read more
Immunohistochemistry-Paraffin: NME4 Antibody (1A5) [NBP2-02487] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-NME4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NME4 Antibody (1A5) [NBP2-02487] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NME4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NME4 Antibody (1A5) [NBP2-02487] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-NME4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NME4 Antibody (1A5) [NBP2-02487] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-NME4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NME4 Antibody (1A5) [NBP2-02487] - Staining of paraffin-embedded Kidney tissue using anti-NME4 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.59 mg/ml

Order Details

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NME4 Antibody (OTI1A5) Summary

Immunogen
Full length human recombinant protein of human NME4 (NP_005000) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NME4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
Theoretical MW
20.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.59 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NME4 Antibody (OTI1A5)

  • EC 2.7.4.6
  • NDP kinase, mitochondrial
  • NDPK-D
  • NM23D
  • nm23-H4mitochondrial
  • non-metastatic cells 4, protein expressed in

Background

The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates.The enzymes are products of the nm23 gene family, which includes NME4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NME4
Entrez