![Western Blot: NKX3.1 Antibody (0361) [NB100-1828] - Analysis of NKX3.1 in HeLa cell lysate using anti-NKX3.1 antibody. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NKX3-1-Antibody-0361-Western-Blot-NB100-1828-img0006.jpg "Western Blot: NKX3.1 Antibody (0361) [NB100-1828] - Analysis of NKX3.1 in HeLa cell lysate using anti-NKX3.1 antibody. WB image submitted by a verified customer review.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, IP, KD |
| Clone | 0361 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 2.0 mg/ml |
| Immunogen | Full-length human Nkx3.1 produced in bacteria as a hexahistidine-tagged protein. [UniProt# Q99801] |
| Localization | Nuclear |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NKX3-1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This Nkx3.1 antibody is useful for Immunohistochemistry frozen and paraffin embedded sections, Immunoprecipitation and Western Blot. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.1% Sodium Azide |
| Concentration | 2.0 mg/ml |
| Purity | Protein G purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IHC-Fr | Human | 08/04/2021 |
Summary
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reviewed by:
Verified Customer |
IHC-P | Mouse | 04/19/2016 |
Summary
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reviewed by:
Ronald Miller |
WB | Human | 11/20/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for NKX3.1 Antibody (NB100-1828)Find related products by research area.
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| Verified Customer 08/04/2021 |
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| Application: | IHC-Fr | |
| Species: | Human |
| Verified Customer 04/19/2016 |
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| Application: | IHC-P | |
| Species: | Mouse |
| Ronald Miller 11/20/2014 |
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| Application: | WB | |
| Species: | Human |
| Gene Symbol | NKX3-1 |
![Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) [NB100-1828] - Staining of human chondrosarcoma tissue with NKX3.1 monoclonal antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NKX3-1-Antibody-0361-Immunohistochemistry-Paraffin-NB100-1828-img0008.jpg "Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) [NB100-1828] - Staining of human chondrosarcoma tissue with NKX3.1 monoclonal antibody. IHC-P image submitted by a verified customer review.")
![Western Blot: Nkx3.1 Antibody (0361) [NB100-1828] - Detection of NKX3.1 in mouse testis lysate using NB 100-1828. ECL detection 1 minute.](http://images.novusbio.com/fullsize/Nkx3-1-Antibody-0361-Western-Blot-NB100-1828-img0003.jpg "Western Blot: Nkx3.1 Antibody (0361) [NB100-1828] - Detection of NKX3.1 in mouse testis lysate using NB 100-1828. ECL detection 1 minute.")
![Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) [NB100-1828] - NKX3.1 was detected in immersion fixed paraffin-embedded sections of mouse testis using Mouse Anti-Mouse NKX3.1 (0361) Monoclonal Antibody (Catalog # NB100-1828) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei in sperm cells.](http://images.novusbio.com/fullsize/NKX3-1-Antibody-0361-Immunohistochemistry-Paraffin-NB100-1828-img0007.jpg "Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) [NB100-1828] - NKX3.1 was detected in immersion fixed paraffin-embedded sections of mouse testis using Mouse Anti-Mouse NKX3.1 (0361) Monoclonal Antibody (Catalog # NB100-1828) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei in sperm cells.")
![Knockdown Validated: NKX3.1 Antibody (0361) [NB100-1828] - DWB analysis of NKX3.1 expression in the indicated cells transfected with Ri-Vector (V-Ri) or shRNAs (Ri#1/2) against NKX3.1. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC6895605/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NKX3-1-Antibody-0361-Knockdown-Validated-NB100-1828-img0009.jpg "Knockdown Validated: NKX3.1 Antibody (0361) [NB100-1828] - DWB analysis of NKX3.1 expression in the indicated cells transfected with Ri-Vector (V-Ri) or shRNAs (Ri#1/2) against NKX3.1. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC6895605/) licensed under a CC-BY license.")
![Western Blot: NKX3.1 Antibody (0361) - BSA Free [NB100-1828] - SALL2 transcriptionally upregulates ESR1 in breast cancerARNA‐seq analysis of ESR1 mRNA levels in 9 paired pre‐tamoxifen‐treated primary breast cancer tissues & relapsed tamoxifen‐resistant breast cancer tissues.BqRT–PCR analysis of ESR1 expression in 9 paired pre‐tamoxifen‐treated primary breast cancer tissues & relapsed tamoxifen‐resistant breast cancer tissues. GAPDH was used as an internal control.C, DWB analysis of SALL2 (A) & NKX3‐1 (B) expression in the indicated cells transfected with Ri‐Vector (V‐Ri) or shRNAs (Ri#1/2) against SALL2 or NKX3‐1. alpha ‐Tubulin was used as the loading control.E, FqRT–PCR analysis of ESR1 expression in the indicated cells transfected with Ri‐Vector or shRNAs (Ri#1/2) against SALL2 or NKX3‐1.Data information: In (A), P‐values were determined by two‐tailed paired Student's t‐test. In (B), data are presented as mean ± SD, & P‐values were determined by two‐tailed unpaired Student's t‐test, n = 3. In (E & F), data are presented as mean ± SD, & P‐values were determined by one‐way ANOVA test, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., no significance. Exact P‐values are specified in Appendix Table S10.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31657150), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1828_mouse-monoclonal-nkx3-1-antibody-0361-310202415525249.jpg "Western Blot: NKX3.1 Antibody (0361) - BSA Free [NB100-1828] - SALL2 transcriptionally upregulates ESR1 in breast cancerARNA‐seq analysis of ESR1 mRNA levels in 9 paired pre‐tamoxifen‐treated primary breast cancer tissues & relapsed tamoxifen‐resistant breast cancer tissues.BqRT–PCR analysis of ESR1 expression in 9 paired pre‐tamoxifen‐treated primary breast cancer tissues & relapsed tamoxifen‐resistant breast cancer tissues. GAPDH was used as an internal control.C, DWB analysis of SALL2 (A) & NKX3‐1 (B) expression in the indicated cells transfected with Ri‐Vector (V‐Ri) or shRNAs (Ri#1/2) against SALL2 or NKX3‐1. alpha ‐Tubulin was used as the loading control.E, FqRT–PCR analysis of ESR1 expression in the indicated cells transfected with Ri‐Vector or shRNAs (Ri#1/2) against SALL2 or NKX3‐1.Data information: In (A), P‐values were determined by two‐tailed paired Student's t‐test. In (B), data are presented as mean ± SD, & P‐values were determined by two‐tailed unpaired Student's t‐test, n = 3. In (E & F), data are presented as mean ± SD, & P‐values were determined by one‐way ANOVA test, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., no significance. Exact P‐values are specified in Appendix Table S10.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31657150), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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