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NKX3.1 Antibody (0361) - Azide and BSA Free

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Western Blot: NKX3.1 Antibody (0361) - Azide and BSA Free [NBP2-80880] - Detection of NKX3.1 in mouse testis lysate using NB 100-1828. ECL detection 1 minute. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) - Azide and BSA Free [NBP2-80880] - NKX3.1 was detected in immersion fixed paraffin-embedded sections of mouse testis using Mouse Anti-Mouse NKX3.1 (0361) Monoclonal ...read more
Western Blot: NKX3.1 Antibody (0361) - Azide and BSA Free [NBP2-80880] - analysis of NKX3.1 in HeLa cell lysate using anti-NKX3.1 antibody. Image from verified customer review. Image from the standard format of this ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP, KD
Clone
0361
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
2.3 mg/ml

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NKX3.1 Antibody (0361) - Azide and BSA Free Summary

Immunogen
Full-length human Nkx3.1 produced in bacteria as a hexahistidine-tagged protein. [UniProt# Q99801]
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NKX3-1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Western Blot 2 ug/ml
Application Notes
This Nkx3.1 antibody is useful for Immunohistochemistry frozen and paraffin embedded sections, Immunoprecipitation and Western Blot.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 22465996).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Concentration
2.3 mg/ml
Purity
Protein G purified

Alternate Names for NKX3.1 Antibody (0361) - Azide and BSA Free

  • BAPX2
  • BAPX2homeobox protein Nkx-3.1
  • Homeobox protein NK-3 homolog A
  • NK homeobox (Drosophila), family 3, A
  • NK3 homeobox 1
  • NK3 transcription factor homolog A
  • NK3 transcription factor related, locus 1 (Drosophila)
  • NK3 transcription factor related, locus 1
  • NKX3.1
  • NKX3.1NKX3
  • NKX3-1
  • NKX3A
  • NKX3ANK homeobox, family 3, A

Background

NKX3.1 (homeobox protein Nkx-3.1) is a homeodomain nuclear transcription factor expressed predominately in the prostate with very low testicular expression and is highly important in prostate biology. NKX3.1 interacts with SRF, SPDEF, WDR77 etc. and TOPORS polyubiquitinates NKX3-1 for inducing its proteasomal degradation regulation. NKX3.1 binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and act as a transcriptional repressor for playing important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Additionally, NKX3.1 functions as a prostate-specific tumor suppressor and diminished NKX3.1 expression is a consistent feature in human/mouse prostate cancers, wherein its expression is lost early in tumorigenesis suggesting that NKX3.1 loss is an early step in the progression to malignant disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NKX3-1