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NGL-1/LRRC4C Antibody

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Western Blot: NGL-1/LRRC4C Antibody [NBP2-19516] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NGL-1/LRRC4C Antibody diluted at 1:2000. HRP-conjugated anti-rabbit ...read more
Immunocytochemistry/ Immunofluorescence: NGL-1/LRRC4C Antibody [NBP2-19516] - Analysis of methanol-fixed MCF-7, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: NGL-1/LRRC4C Antibody [NBP2-19516] - Human breast cancer, using Netrin G1 ligand antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: NGL-1/LRRC4C Antibody [NBP2-19516] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:2000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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NGL-1/LRRC4C Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human NGL-1/LRRC4C. The exact sequence is proprietary.
Localization
Membrane; Single-pass type I membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LRRC4C
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris-Glycine (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for NGL-1/LRRC4C Antibody

  • KIAA1580NGL-1NGL1Netrin-G1 ligand
  • leucine rich repeat containing 4C
  • leucine-rich repeat-containing protein 4C
  • LRRC4C
  • NGL1
  • NGL-1

Background

NGL1 is a specific binding partner for netrin G1 (NTNG1; MIM 608818), which is a member of the netrin family of axon guidance molecules (Lin et al., 2003 [PubMed 14595443]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol LRRC4C
Uniprot