![Western Blot: CDKL5 Antibody [NBP2-32157] - Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody NBP2-32157 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDKL5-Antibody-Western-Blot-NBP2-32157-img0001.jpg "Western Blot: CDKL5 Antibody [NBP2-32157] - Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody NBP2-32157 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen this antibody was made to, maps to a region between residue 350 to 400 of human Cyclin-Dependent Kinase-Like 5 using the numbering given in entry NP_003150.1 (GeneID 6792). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CDKL5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for CDKL5 Antibody (NBP2-32157)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CDKL5 |
| Uniprot |
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![Immunohistochemistry-Paraffin: CDKL5 Antibody [NBP2-32157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- CDKL5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/CDKL5-Antibody-Immunohistochemistry-Paraffin-NBP2-32157-img0006.jpg "Immunohistochemistry-Paraffin: CDKL5 Antibody [NBP2-32157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- CDKL5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).")
![Immunohistochemistry-Paraffin: CDKL5 Antibody [NBP2-32157] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- CDKL5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/CDKL5-Antibody-Immunohistochemistry-Paraffin-NBP2-32157-img0005.jpg "Immunohistochemistry-Paraffin: CDKL5 Antibody [NBP2-32157] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- CDKL5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).")
![Immunoprecipitation: CDKL5 Antibody [NBP2-32157] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody NBP2-32157 used for IP at 6 ug/mg lysate. CDKL5 was inefficiently immunoprecipitated by rabbit anti-CDKL5 antibody BL15151. For blotting immunoprecipitated CDKL5, NBP2-32157 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDKL5-Antibody-Immunoprecipitation-NBP2-32157-img0002.jpg "Immunoprecipitation: CDKL5 Antibody [NBP2-32157] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody NBP2-32157 used for IP at 6 ug/mg lysate. CDKL5 was inefficiently immunoprecipitated by rabbit anti-CDKL5 antibody BL15151. For blotting immunoprecipitated CDKL5, NBP2-32157 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
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![Immunohistochemistry Netrin-G1a Antibody [Unconjugated]](https://images.novusbio.com/images/af1166_mouse-netrin-g1a-affinity-purified-polyclonal-ab-8220249503271.jpg)
![Immunocytochemistry/ Immunofluorescence Netrin-G1a Antibody [Unconjugated]](https://images.novusbio.com/images/af1166_mouse-netrin-g1a-affinity-purified-polyclonal-ab-8220249503240.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")