![Western Blot: NF-H Antibody (AH1) [NBP1-05209] - Analysis of the heavily phosphorylated axonal form of NF-H protein (pNF-H) in neural tissue lysates (20ug/lane) with affinity purified mouse monoclonal anti-pNF-H antibody (NBP1-05209) at dilution of 1:5,000. Lanes on the blot are: [1] Protein size marker, [2] Adult rat whole brain [3] Embryonic (E20) rat whole brain [4] Adult rat spinal cord [5] Adult mouse whole brain [6] Adult mouse spinal cord. Rodent pNF-H protein appears as a single band of about 200kDa in adult rat and mouse lysates, but is not present in early development (Lane 3). Additional bands appearing on the blot (Lane 4) are most likely partially degraded products of pNF-H protein.](http://images.novusbio.com/fullsize/NF-H-Antibody-AH1-Western-Blot-NBP1-05209-img0005.jpg "Western Blot: NF-H Antibody (AH1) [NBP1-05209] - Analysis of the heavily phosphorylated axonal form of NF-H protein (pNF-H) in neural tissue lysates (20ug/lane) with affinity purified mouse monoclonal anti-pNF-H antibody (NBP1-05209) at dilution of 1:5,000. Lanes on the blot are: [1] Protein size marker, [2] Adult rat whole brain [3] Embryonic (E20) rat whole brain [4] Adult rat spinal cord [5] Adult mouse whole brain [6] Adult mouse spinal cord. Rodent pNF-H protein appears as a single band of about 200kDa in adult rat and mouse lysates, but is not present in early development (Lane 3). Additional bands appearing on the blot (Lane 4) are most likely partially degraded products of pNF-H protein.")
| Reactivity | Hu, Mu, Rt, Po, Bv, EqSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC |
| Clone | AH1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Native axonal phosphorylated Neurofilament Heavy protein purified from bovine spinal cord |
| Marker | Neuronal Marker |
| Specificity | Clone AH1 recognizes phosphorylated NF-H KSP sequences. Does not recognize non-phosphorylated KSP sequences. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NEFH |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This 200kDa Neurofilament Heavy antibody is useful for Imunocytochemistry, Western Blot, and Immunohistochemistry (both IHC-P and IHC-Fr). Use in IHC-P reported in scientific literature (PMID:35835391). |
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| Theoretical MW | 200 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol |
| Preservative | 0.035% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Affinity purified |
| Publication using NBP1-05209 | Applications | Species |
|---|---|---|
| Duan S, Sawyer TW, Sontz RA et al. GFAP-directed Inactivation of Men1 Exploits Glial Cell Plasticity in Favor of Neuroendocrine Reprogramming Cellular and molecular gastroenterology and hepatology 2022-07-11 [PMID: 35835391] (IHC-P, Mouse) | IHC-P | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for NF-H Antibody (NBP1-05209)Find related products by research area.
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Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: NF-H Antibody (AH1) [NBP1-05209] - Mixed neuron/glial cultures stained with NBP1-05209 (green) and also stained with rabbit polyclonal antibody to neurofilament NF-L NB300-131 (red). The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while NBP1-05209 binds to only heavily phosphorylated forms of NF-H which are localized to mature axonal neurofilaments. In this image a few axons course from left to right and top to bottom since they contain both NF-L and phosphorylated NF-H they appear golden in color. Blue shows the distribution of DNA.](http://images.novusbio.com/fullsize/NF-H-Antibody-AH1-Immunocytochemistry-Immunofluorescence-NBP1-05209-img0003.jpg "Immunocytochemistry/Immunofluorescence: NF-H Antibody (AH1) [NBP1-05209] - Mixed neuron/glial cultures stained with NBP1-05209 (green) and also stained with rabbit polyclonal antibody to neurofilament NF-L NB300-131 (red). The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while NBP1-05209 binds to only heavily phosphorylated forms of NF-H which are localized to mature axonal neurofilaments. In this image a few axons course from left to right and top to bottom since they contain both NF-L and phosphorylated NF-H they appear golden in color. Blue shows the distribution of DNA.")
![Immunohistochemistry Free-Floating: NF-H Antibody (AH1) [NBP1-05209] - Analysis of rat cerebellum section stained with mouse mAb to pNF-H, NBP1-05209, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN, dilution 1:5,000 in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-05209 antibody stains axons in the granular layer and white matter and prominent basket cell axons surrounding the large Purkinje neurons. The FOX3/NeuN antibody specifically labels nuclei of granular and other neurons, but does not stain Purkinje cells.](http://images.novusbio.com/fullsize/NF-H-Antibody-AH1-Immunocytochemistry-Immunofluorescence-NBP1-05209-img0004.jpg "Immunohistochemistry Free-Floating: NF-H Antibody (AH1) [NBP1-05209] - Analysis of rat cerebellum section stained with mouse mAb to pNF-H, NBP1-05209, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN, dilution 1:5,000 in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-05209 antibody stains axons in the granular layer and white matter and prominent basket cell axons surrounding the large Purkinje neurons. The FOX3/NeuN antibody specifically labels nuclei of granular and other neurons, but does not stain Purkinje cells.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")