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Nestin Antibody (4D11) [Janelia Fluor® 635]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
4D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 635

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Nestin Antibody (4D11) [Janelia Fluor® 635] Summary

Immunogen
Recombinant construct, amino acids 317-630 of the human Nestin protein expressed in and purified from E. coli. [UniProt P48681]
Marker
Neural Stem Cell Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NES
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Nestin Antibody (4D11) [Janelia Fluor® 635]

  • FLJ21841
  • NES
  • Nestin

Background

Nestin is a Class VI intermediate filament expressed in the developing central nervous system in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Because if it's roles in angiogenisis and tumorgenesis, Nestin antibodies are useful cancer markers.

Although it is often used as a marker for proliferating and migrating cells very little is known about Nestin's function or regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nestin Antibody (NBP1-92717JF635) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nestin Antibody (NBP1-92717JF635) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Nestin Products

Research Areas for Nestin Antibody (NBP1-92717JF635)

Find related products by research area.

Blogs on Nestin.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including ...  Read full blog post.

mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol NES