Nestin Antibody (307501) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Nestin is a class VI intermediate filament protein (1,2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells.(4) Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway.(2) Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-P, Immunocytochemistry, Western Blot.

Showing Publications 1 - 10 of 28. Show All 28 Publications. Collapse Publications.

Publications using MAB2736	Applications	Species
Jager, J;Ribeiro, M;Furtado, M;Carvalho, T;Syrris, P;Lopes, LR;Elliott, PM;Cabral, JMS;Carmo-Fonseca, M;da Rocha, ST;Martins, S; Patient-derived induced pluripotent stem cells to study non-canonical splicing variants associated with Hypertrophic Cardiomyopathy Stem cell research 2024-10-16 [PMID: 39447317] (Immunocytochemistry, Human)	Immunocytochemistry	Human
Ribeiro, M;Martins, S;Carvalho, T;Furtado, M;Sampaio Cabral, J;Brito, D;Carmo-Fonseca, M;da Rocha, ST; Generation of induced pluripotent stem cell lines from two unrelated individuals with familial hypertrophic cardiomyopathy carrying the MYBPC3 missense c.1484G>A mutation Stem cell research 2023-12-13 [PMID: 38104429] (ICC, Human)	ICC	Human
H Kayir, BW Jenkins, B Alural, JY Khokhar Clozapine Increases Nestin Concentration in the Adult Male Rat Hippocampus: A Preliminary Study International Journal of Molecular Sciences, 2022-03-22;23(7):. 2022-03-22 [PMID: 35408792] (Western Blot, Mouse)	Western Blot	Mouse
C Maranga, C Pereira, AC Raposo, A Vieira, S Duarte, EP Bekman, I Milagre, ST da Rocha Generation and characterization of induced pluripotent stem cell line (IBBISTi004-A) from an Angelman syndrome patient carrying a class II deletion of the maternal chromosome 15q11.2-q13 Stem Cell Research, 2022-03-21;61(0):102757. 2022-03-21 [PMID: 35339881] (ICC, Human)	ICC	Human
Francesca Ciarpella, Raluca Georgiana Zamfir, Alessandra Campanelli, Elisa Ren, Giulia Pedrotti, Emanuela Bottani et al. Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity iScience 2021-12-17 [PMID: 34901791]
Zachary T. Olmsted, Cinzia Stigliano, Annalisa Scimemi, Tatiana Wolfe, Jose Cibelli, Philip J. Horner et al. Transplantable human motor networks as a neuron-directed strategy for spinal cord injury iScience 2021-08-20 [PMID: 34381965]
A Ohyama, A Osada, H Kawaguchi, I Kurata, T Nishiyama, T Iwai, A Ishigami, Y Kondo, H Tsuboi, T Sumida, I Matsumoto Specific Increase in Joint Neutrophil Extracellular Traps and Its Relation to Interleukin 6 in Autoimmune Arthritis International Journal of Molecular Sciences, 2021-07-16;22(14):. 2021-07-16 [PMID: 34299252] (IHC, Mouse)	IHC	Mouse
Eunyoung Park, Johnathan G. Lyon, Melissa Alvarado‐Velez, Martha I. Betancur, Nassir Mokarram, Jennifer H. Shin et al. Enriching neural stem cell and anti‐inflammatory glial phenotypes with electrical stimulation after traumatic brain injury in male rats Journal of Neuroscience Research 2021-07-01 [PMID: 33772860]
Jing-Xia Liu, Fatima Pedrosa Domellöf Cytoskeletal Proteins in Myotendinous Junctions of Human Extraocular Muscles Investigative Opthalmology & Visual Science 2021-02-17 [PMID: 33595614]
NS Singhal, M Bai, EM Lee, S Luo, KR Cook, DK Ma Cytoprotection by a naturally occurring variant of ATP5G1 in Arctic ground squirrel neural progenitor cells Elife, 2020-10-14;9(0):. 10/14/2020 [PMID: 33050999]
K Tsuji, S Kitamura, Y Sang, K Fukushima, J Wada Adult kidney stem/progenitor cells contribute to regeneration through the secretion of trophic factors Stem Cell Res, 2020-05-31;46(0):101865. 2020-05-31 [PMID: 32505897] (IHC, Rat)	IHC	Rat
HN Robeson, HR Lau, LA New, J Lalonde, JN Armstrong, N Jones Localization of phosphotyrosine adaptor protein ShcD/SHC4 in the adult rat central nervous system BMC Neurosci, 2019-12-10;20(1):57. 2019-12-10 [PMID: 31823725] (IHC, Rat)	IHC	Rat
Marwah Abd Al Samid, Jamie S. McPhee, Jasdeep Saini, Tristan R. McKay, Lorna M. Fitzpatrick, Kamel Mamchaoui et al. A functional human motor unit platform engineered from human embryonic stem cells and immortalized skeletal myoblasts Stem Cells and Cloning: Advances and Applications 2018-12-07 [PMID: 30519053]
WS Bang, KT Kim, YJ Seo, DC Cho, JK Sung, CH Kim Curcumin Increase the Expression of Neural Stem/Progenitor Cells and Improves Functional Recovery after Spinal Cord Injury J Korean Neurosurg Soc, 2017-12-29;61(1):10-18. 2017-12-29 [PMID: 29354231] (ICC, IHC, Rat)	ICC, IHC	Rat
A Yoshimura, N Adachi, H Matsuno, M Kawamata, Y Yoshioka, H Kikuchi, H Odaka, T Numakawa, H Kunugi, T Ochiya, Y Tamai The Sox2 promoter-driven CD63-GFP transgenic rat model allows tracking neural stem cell-derived extracellular vesicles Dis Model Mech, 2018-01-30;0(0):. 2018-01-30 [PMID: 29208635] (ICC, Rat)	ICC	Rat
N Hamada, I Iwamoto, H Tabata, KI Nagata MUNC18-1 gene abnormalities are involved in neurodevelopmental disorders through defective cortical architecture during brain development Acta Neuropathol Commun, 2017-11-30;5(1):92. 2017-11-30 [PMID: 29191246] (ICC, Primate)	ICC	Primate
P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia Oncotarget, 2017-04-25;8(17):28074-28092. 2017-04-25 [PMID: 28427209] (ICC, Western Blot, Human)	ICC, Western Blot	Human
NM Thellman, C Botting, Z Madaj, SJ Triezenber An Immortalized Human Dorsal Root Ganglia Cell Line Provides a Novel Context to Study Herpes Simplex Virus Type-1 Latency and Reactivation J. Virol., 2017-05-26;0(0):. 2017-05-26 [PMID: 28404842] (ICC, Human)	ICC	Human
SB Ross, ST Fraser, RD Bagnall, C Semsarian Peripheral blood derived induced pluripotent stem cells (iPSCs) from a female with familial hypertrophic cardiomyopathy Stem Cell Res, 2017-03-07;20(0):76-79. 2017-03-07 [PMID: 28395744] (IHC, Human)	IHC	Human
Maria Fernanda Forni, Aline Ramos Maia Lobba, Alexandre Hamilton Pereira Ferreira, Mari Cleide Sogayar Simultaneous Isolation of Three Different Stem Cell Populations from Murine Skin PLOS ONE 2015-10-13 [PMID: 26462205]
Huang B, Zhan Y, Zong W, Yu C, Li J, Qu Y, Han S The PD-1/B7-H1 pathway modulates the natural killer cells versus mouse glioma stem cells. PLoS ONE, 2015-08-12;10(8):e0134715. 2015-08-12 [PMID: 26266810] (ICC, Mouse)	ICC	Mouse
Lyu J, Yu X, He L, Cheng T, Zhou J, Cheng C, Chen Z, Cheng G, Qiu Z, Zhou W The protein phosphatase activity of PTEN is essential for regulating neural stem cell differentiation. Mol Brain, 2015-04-18;8(0):26. 2015-04-18 [PMID: 25927309] (IHC, Mouse)	IHC	Mouse
Engels M, Rajarajan K, Feistritzer R, Sharma A, Nielsen U, Schalij M, de Vries A, Pijnappels D, Wu S Insulin-like growth factor promotes cardiac lineage induction in vitro by selective expansion of early mesoderm. Stem Cells, 2014-06-01;32(6):1493-502. 2014-06-01 [PMID: 24496962] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Narita K, Matsuda Y, Seike M, Naito Z, Gemma A, Ishiwata T Nestin regulates proliferation, migration, invasion and stemness of lung adenocarcinoma. Int J Oncol, 2014-01-24;44(4):1118-30. 2014-01-24 [PMID: 24481417] (IHC-P, Human)	IHC-P	Human
Matsuda Y, Yoshimura H, Ueda J, Naito Z, Korc M, Ishiwata T Nestin delineates pancreatic cancer stem cells in metastatic foci of NOD/Shi-scid IL2Rgamma(null) (NOG) mice. Am J Pathol, 2014-01-09;184(3):674-85. 2014-01-09 [PMID: 24412093] (Flow Cytometry, IHC-P, Mouse)	Flow Cytometry, IHC-P	Mouse
Koyama-Nasu , R, Haruta , R, Nasu-Nishimura , Y, Taniue , K, Katou , Y, Shirahige , K, Todo , T, Ino , Y, Mukasa , A, Saito , N, Matsui , M, Takahashi , R, Hoshino-Okubo , A, Sugano , H, Manabe , E, Funato , K, Akiyama , T The pleiotrophin-ALK axis is required for tumorigenicity of glioblastoma stem cells. Oncogene, 2013-05-20;33(17):2236-44. 2013-05-20 [PMID: 23686309] (Western Blot, Human)	Western Blot	Human
Mutnal MB, Cheeran MC, Hu S Murine cytomegalovirus infection of neural stem cells alters neurogenesis in the developing brain. PLoS ONE, 2011-01-13;6(1):e16211. 2011-01-13 [PMID: 21249143] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Sun J, Sha B, Zhou W, Yang Y VEGF-mediated angiogenesis stimulates neural stem cell proliferation and differentiation in the premature brain. Biochem. Biophys. Res. Commun., 2010-02-24;394(1):146-52. 2010-02-24 [PMID: 20188072] (ICC, Western Blot, Rat)	ICC, Western Blot	Rat
Show All 28 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	ICC	Rat	02/19/2015	View Summary Application Immunocytochemistry Sample Tested See PMID 23798370 Species Rat

Array MAB2736

• Nestin Antibodies
• Nestin ELISA Kits
• Nestin Proteins