![Western Blot: Nesprin 2 Antibody (10H8) [NBP2-59945] - HEK293 cells transiently expressing either GFP alone or GFP fused a small Nesp2 isoform (Nesp2a2DKASH) lacking the C-terminal KASH domain. This small isoform contains the ~180 residue Nesp2 polypeptide that was employed in the immunogen. Total cell lysates were analyzed by western blot employing either a rabbit anti-GFP or culture supernatant from three independent anti-Nesp2 hybridoma clones (6C11, 10H8 and 5C3)](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-10H8-Western-Blot-NBP2-59945-img0001.jpg "Western Blot: Nesprin 2 Antibody (10H8) [NBP2-59945] - HEK293 cells transiently expressing either GFP alone or GFP fused a small Nesp2 isoform (Nesp2a2DKASH) lacking the C-terminal KASH domain. This small isoform contains the ~180 residue Nesp2 polypeptide that was employed in the immunogen. Total cell lysates were analyzed by western blot employing either a rabbit anti-GFP or culture supernatant from three independent anti-Nesp2 hybridoma clones (6C11, 10H8 and 5C3)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | 10H8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | GST fused to residues 6,653-6,835 of human Nesprin 2 |
| Specificity | Specificity of human Nesprin 2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SYNE2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | SYNE2 |
![Immunocytochemistry/Immunofluorescence: Nesprin 2 Antibody (10H8) [NBP2-59945] - Staining in HeLa cells labeled with either 6C11 or 10H8 monoclonal antibodies raised against human Nesp2. The antibodies can be used on either formaldehyde- or methanol-fixed cells.](http://images.novusbio.com/fullsize/Nesprin-2-Antibody-10H8-Immunocytochemistry-Immunofluorescence-NBP2-59945-img0002.jpg "Immunocytochemistry/Immunofluorescence: Nesprin 2 Antibody (10H8) [NBP2-59945] - Staining in HeLa cells labeled with either 6C11 or 10H8 monoclonal antibodies raised against human Nesp2. The antibodies can be used on either formaldehyde- or methanol-fixed cells.")
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