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NEDD4L Antibody (1D2)

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Western Blot: NEDD4L Antibody (1D2) [H00023327-M04] - Analysis of NEDD4L expression in transfected 293T cell line by NEDD4L monoclonal antibody (M04), clone 1D2. Lane 1: NEDD4L transfected lysate (Predicted MW: 104.9 KDa). Lane 2: Non-transfected lysate.
Western Blot: NEDD4L Antibody (1D2) [H00023327-M04] - Analysis of NEDD4L expression in transfected 293T cell line by NEDD4L monoclonal antibody (M04), clone 1D2. Lane 1: NEDD4L transfected lysate (Predicted MW: 104.9 ...read more
Immunoprecipitation: NEDD4L Antibody (1D2) [H00023327-M04] - Analysis of NEDD4L transfected lysate using anti-NEDD4L monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEDD4L monoclonal antibody.
Sandwich ELISA: NEDD4L Antibody (1D2) [H00023327-M04] - Detection limit for recombinant GST tagged NEDD4L is 0.3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP
Clone
1D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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NEDD4L Antibody (1D2) Summary

Immunogen
NEDD4L (AAH32597, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATGLGEPVYGLSEDEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLT
Specificity
NEDD4L (1D2)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NEDD4L
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NEDD4L Antibody (1D2)

  • E3 ubiquitin-protein ligase NEDD4-like
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ33870
  • hNedd4-2
  • KIAA0439ubiquitin-protein ligase NEDD4-like
  • NEDD4.2
  • NEDD4-2
  • NEDL3
  • neural precursor cell expressed, developmentally down-regulated 4-like
  • neural precursor cell expressed, developmentally down-regulated gene 4-like
  • RSP5ubiquitin-protein ligase Rsp5

Background

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00023327-M04
NEDD4L Antibody (1D2)
Species: Hu
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NEDD4L
Entrez
OMIM
Uniprot