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NDP52 Antibody

Images

 
Western Blot: NDP52 Antibody [H00010241-B01P] - Hypothalamic autophagy at day 17. (c) Representative immunoblots (from 3 individuals/group) and expression profile (n = 6/group) of BNIP3 and NDP52 at d17 in the ...read more
Western Blot: NDP52 Antibody [H00010241-B01P] - CALCOCO2 MaxPab polyclonal antibody. Western Blot analysis of CALCOCO2 expression in human pancreas.
Western Blot: NDP52 Antibody [H00010241-B01P] - Analysis of CALCOCO2 expression in human liver.
Western Blot: NDP52 Antibody [H00010241-B01P] - Analysis of CALCOCO2 expression in transfected 293T cell line by CALCOCO2 polyclonal antibody. Lane 1: CALCOCO2 transfected lysate(49.06 KDa). Lane 2: Non-transfected ...read more
Western Blot: NDP52 Antibody [H00010241-B01P] - Analysis of CALCOCO2 expression in human pancreas.
Western Blot: NDP52 Antibody [H00010241-B01P] - Hypothalamic autophagy at day 17. (a) Representative immunoblots (n = 1/group) & expression profile (n = 6/group) of LC3I, LC3II at d17 (Supplemental Fig. S8) & ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

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NDP52 Antibody Summary

Immunogen
CALCOCO2 (NP_005822.1, 1 a.a. - 446 a.a.) full-length human protein. MEETIKDPPTSAVLLDHCHFSQVIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYYTFMWVTLPIDLNNKSAKQQEVQFKAYYLPKDDEYYQFCYVDEDGVVRGASIPFQFRPENEEDILVVTTQGEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETLQSINKKLELKVKEQKDYWETELLQLKEQNQKMSSENEKMGIRVDQLQAQLSTQEKEMEKLVQGDQDKTEQLEQLKKENDHLFLSLTEQRKDQKKLEQTVEQMKQNETTAMKKQQELMDENFDLSKRLSENEIICNALQRQKERLEGENDLLKRENSRLLSYMGLDFNSLPYQVPTSDEGGARQNPGLAYGNPYSGIQESSSPSPLSIKKCPICKADDICDHTLEQQQMQPLCFNCPICDKIFPATEKQIFEDHVFCHSL
Specificity
CALCOCO2 - calcium binding and coiled-coil domain 2,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
CALCOCO2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control). Use in ICC/IF was reported in scientific literature (PMID: 31611642).
Publications
Read Publications using
H00010241-B01P in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24667209)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NDP52 Antibody

  • Antigen nuclear dot 52 kDa protein
  • calcium binding and coiled-coil domain 2
  • calcium-binding and coiled-coil domain-containing protein 2
  • NDP52MGC17318
  • Nuclear domain 10 protein 52
  • Nuclear domain 10 protein NDP52
  • Nuclear dot protein 52

Background

The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00010241-B01P
Species: Hu, Mu
Applications: WB, ICC/IF

Publications for NDP52 Antibody (H00010241-B01P)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(3)
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Human
(1)
Mouse
(4)
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Showing Publications 1 - 10 of 10.
Publications using H00010241-B01P Applications Species
Kibrom A, Yaritza D, Rosemary C et al. Inner mitochondrial membrane protein Prohibitin 1 mediates Nix-induced, Parkin-independent mitophagy. Sci Rep. 2023-01-02 [PMID: 36593241]
Borgo GM, Burke TP, Tran CJ et al. A patatin-like phospholipase mediates Rickettsia parkeri escape from host membranes Nature communications 2022-06-27 [PMID: 35760786]
Engstr�m P, Burke TP, Tran CJ et al. Lysine methylation shields an intracellular pathogen from ubiquitylation and autophagy Science Advances 2021-06-25 [PMID: 34172444]
Cara E, Wanda K, Keith B et al. Transbilayer Movement of Sphingomyelin Precedes Catastrophic Breakage of Enterobacteria-Containing Vacuoles. Curr Biol. 2020-06-26 [PMID: 32649908]
EngstrOm P, Burke TP, Mitchell G et al. Evasion of autophagy mediated by Rickettsia surface protein OmpB is critical for virulence Nat Microbiol 2019-10-14 [PMID: 31611642] (ICC/IF, Human, Mouse) ICC/IF Human, Mouse
Ravenhill BJ, Boyle KB, von Muhlinen N et al. The Cargo Receptor NDP52 Initiates Selective Autophagy by Recruiting the ULK Complex to Cytosol-Invading Bacteria. Mol Cell. 2019-03-07 [PMID: 30853402]
Pabon MA, Patino E, Bhatia D et al. Beclin-1 regulates cigarette smoke-induced kidney injury in a murine model of chronic obstructive pulmonary disease. JCI Insight 2018-09-20 [PMID: 30232271] (WB, Mouse) WB Mouse
Nobis S, Goichon A, Achamrah N et al. Alterations of proteome, mitochondrial dynamic and autophagy in the hypothalamus during activity-based anorexia Sci Rep 2018-05-08 [PMID: 29740148] (WB, Mouse) WB Mouse
Kim S, Lee D, Song JC et al. NDP52 associates with phosphorylated tau in brains of an Alzheimer disease mouse model. Biochem Biophys Res Commun 2014-11-07 [PMID: 25450380]
Jo C, Gundemir S, Pritchard S et al. Nrf2 Reduces Levels of Phosphorylated Tau Protein by Inducing Autophagy Adaptor Protein NDP52 Nat Commun. 2014-03-25 [PMID: 24667209] (WB, Mouse) WB Mouse

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional NDP52 Products

Blogs on NDP52.

Understanding Mitophagy Mechanisms: Canonical PINK1/Parkin, LC3-Dependent Piecemeal, and LC3-Independent Mitochondrial Derived Vesicles
By Christina Towers, PhD What is Mitophagy?The selective degradation of mitochondria via double membrane autophagosome vesicles is called mitophagy. Damaged mitochondria can generate harmful amounts of reactive ox...  Read full blog post.

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Bioinformatics

Gene Symbol CALCOCO2
Entrez
Uniprot