![Western Blot: NDP52 Antibody (4H5) [NBP2-03246] Analysis of extracts (35ug) from 9 different cell lines by using anti-NDP52 monoclonal antibody.](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Western-Blot-NBP2-03246-img0006.jpg "Western Blot: NDP52 Antibody (4H5) [NBP2-03246] Analysis of extracts (35ug) from 9 different cell lines by using anti-NDP52 monoclonal antibody.")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clone | OTI4H5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human CALCOCO2 (NP_005822) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CALCOCO2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 52.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP2-03246 | Applications | Species |
|---|---|---|
| Kono Y, Colley T, To M, et al. Cigarette smoke-induced impairment of autophagy in macrophages increases galectin-8 and inflammation Scientific reports 2021-01-11 [PMID: 33432024] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
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Understanding Mitophagy Mechanisms: Canonical PINK1/Parkin, LC3-Dependent Piecemeal, and LC3-Independent Mitochondrial Derived Vesicles By Christina Towers, PhD What is Mitophagy?The selective degradation of mitochondria via double membrane autophagosome vesicles is called mitophagy. Damaged mitochondria can generate harmful amounts of reactive ox... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: NDP52 Antibody (4H5) [NBP2-03246] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NDP52.](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Immunocytochemistry-Immunofluorescence-NBP2-03246-img0005.jpg "Immunocytochemistry/Immunofluorescence: NDP52 Antibody (4H5) [NBP2-03246] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NDP52.")
![Flow Cytometry: NDP52 Antibody (4H5) [NBP2-03246] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NDP52 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Flow-Cytometry-NBP2-03246-img0003.jpg "Flow Cytometry: NDP52 Antibody (4H5) [NBP2-03246] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NDP52 antibody, and then analyzed by flow cytometry.")
![Western Blot: NDP52 Antibody (4H5) [NBP2-03246] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDP52 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDP52.](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Western-Blot-NBP2-03246-img0004.jpg "Western Blot: NDP52 Antibody (4H5) [NBP2-03246] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDP52 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDP52.")
![Flow Cytometry: NDP52 Antibody (4H5) [NBP2-03246] - Analysis of Hela cells, using anti-NDP52 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Flow-Cytometry-NBP2-03246-img0001.jpg "Flow Cytometry: NDP52 Antibody (4H5) [NBP2-03246] - Analysis of Hela cells, using anti-NDP52 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Flow Cytometry: NDP52 Antibody (4H5) [NBP2-03246] - Analysis of Jurkat cells, using anti-NDP52 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Flow-Cytometry-NBP2-03246-img0002.jpg "Flow Cytometry: NDP52 Antibody (4H5) [NBP2-03246] - Analysis of Jurkat cells, using anti-NDP52 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Western Blot Galectin-8 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin8_AF1305_Western_Blot_16880.jpg)
![Simple Western Galectin-8 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin8_AF1305_Simple_Western_18662.jpg)
![Immunohistochemistry Galectin-8 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin8_AF1305_Immunohistochemistry_19141.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")