![Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT1813].](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Western-Blot-NBP2-43588-img0007.jpg "Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT1813].")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | 1813 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary. |
| Predicted Species | Chimpanzee (97%). Backed by our 100% Guarantee. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CALCOCO2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 52 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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Understanding Mitophagy Mechanisms: Canonical PINK1/Parkin, LC3-Dependent Piecemeal, and LC3-Independent Mitochondrial Derived Vesicles By Christina Towers, PhD What is Mitophagy?The selective degradation of mitochondria via double membrane autophagosome vesicles is called mitophagy. Damaged mitochondria can generate harmful amounts of reactive ox... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CALCOCO2 |
![Immunocytochemistry/Immunofluorescence: NDP52 Antibody (1813) [NBP2-43588] - Analysis of Samples: HeLa cells mock (left) and treated with 50uM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 protein stained by NDP52 antibody [1813]. Red: Phalloidin, a F-actin marker.](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Immunocytochemistry-Immunofluorescence-NBP2-43588-img0006.jpg "Immunocytochemistry/Immunofluorescence: NDP52 Antibody (1813) [NBP2-43588] - Analysis of Samples: HeLa cells mock (left) and treated with 50uM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 protein stained by NDP52 antibody [1813]. Red: Phalloidin, a F-actin marker.")
![Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of A. 30 ug Huh7 whole cell lysate/extract B. 30 ug Hep3B whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [1813] dilution: 1:1000](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Western-Blot-NBP2-43588-img0001.jpg "Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of A. 30 ug Huh7 whole cell lysate/extract B. 30 ug Hep3B whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [1813] dilution: 1:1000")
![Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug NCI-H929 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [1813] dilution: 1:1000.](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Western-Blot-NBP2-43588-img0002.jpg "Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug NCI-H929 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [1813] dilution: 1:1000.")
![Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody. The ACTB was used as an internal control (bottom panel).](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Western-Blot-NBP2-43588-img0003.jpg "Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody. The ACTB was used as an internal control (bottom panel).")
![Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. Huh-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody. ACTB was used as internal control (bottom panel).](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Western-Blot-NBP2-43588-img0005.jpg "Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. Huh-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody. ACTB was used as internal control (bottom panel).")
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