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Recombinant Human NCF1 His Protein

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SDS-Page: Recombinant Human NCF1 His Protein [NBP1-45305] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

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Recombinant Human NCF1 His Protein Summary

Description
A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-390 of Human NCF1

Source: E.coli

Amino Acid Sequence: MGDTFIRHIA LLGFEKRFVP SQHYVYMFLV KWQDLSEKVV YRRFTEIYEF HKTLKEMFPI EAGAINPENR IIPHLPAPKW FDGQRAAENR QGTLTEYCST LMSLPTKISR CPHLLDFFKV RPDDLKLPTD NQTKKPETYL MPKDGKSTAT DITGPIILQT YRAIANYEKT SGSEMALSTG DVVEVVEKSE SGWWFCQMKA KRGWIPASFL EPLDSPDETE DPEPNYAGEP YVAIKAYTAV EGDEVSLLEG EAVEVIHKLL DGWWVIRKDD VTGYFPSMYL QKSGQDVSQA QRQIKRGAPP RRSSIRNAHS IHQRSRKRLS QDAYRRNSVR FLQQRRRQAR PGPQSPGSPL EEERQTQRSK PQPAVPPRPS ADLILNRCSE STKRKLASAV VEHHHHHH

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
NCF1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
45.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 20% glycerol, 0.1 M NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human NCF1 His Protein

  • 47 kDa neutrophil oxidase factor
  • FLJ79451
  • NADPH oxidase organizer 2
  • NCF-1
  • NCF1A
  • neutrophil cytosol factor 1,47 kDa autosomal chronic granulomatous disease protein
  • neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal1)
  • neutrophil cytosolic factor 1
  • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1)
  • Neutrophil NADPH oxidase factor 1
  • Nox organizer 2
  • NOXO2NCF-47K
  • Nox-organizing protein 2
  • p47phox
  • SH3 and PX domain-containing protein 1A
  • SH3PXD1Ap47-phox

Background

Neutrophil cytosolic factor 1, also known as NCF1, is a cytosolic subunit of neutrophil NADPH oxidase. NCF1, along with NCF2 and a membrane bound cytochrome b558, is required for activation of the latent NADPH oxidase necessary for superoxide production. Defects in NCF1, often resulting from recombination between NCF1 and a nearby homologous pseudogene, cause chronic granulomatous disease. Recombinant human NCF1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Identifying tumoral and stromal transcriptomes that underlie tumor plasticity and stromal neuroinflammatory response in brain metastasis
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Bioinformatics

Gene Symbol NCF1