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Nab2 Antibody (OTI5D7) - Azide and BSA Free

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Western Blot: Nab2 Antibody (OTI5D7) - Azide and BSA Free [NBP2-72868] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAB2.
Western Blot: Nab2 Antibody (OTI5D7) - Azide and BSA Free [NBP2-72868] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAB2.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI5D7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72868AF350
Alexa Fluor 405
NBP2-72868AF405
Alexa Fluor 488
NBP2-72868AF488
Alexa Fluor 532
NBP2-72868AF532
Alexa Fluor 594
NBP2-72868AF594
Alexa Fluor 647
NBP2-72868AF647
Alexa Fluor 700
NBP2-72868AF700
Alexa Fluor 750
NBP2-72868AF750
Allophycocyanin
NBP2-72868APC
Biotin
NBP2-72868B
CoraFluor 1
NBP2-72868CL1
DyLight 350
NBP2-72868UV
DyLight 405
NBP2-72868V
DyLight 488
NBP2-72868G
DyLight 550
NBP2-72868R
DyLight 594
NBP2-72868DL594
DyLight 650
NBP2-72868C
DyLight 680
NBP2-72868FR
DyLight 755
NBP2-72868IR
FITC
NBP2-72868F
HRP
NBP2-72868H
Janelia Fluor 525
NBP2-72868JF525
Janelia Fluor 549
NBP2-72868JF549
Janelia Fluor 646
NBP2-72868JF646
Janelia Fluor 669
NBP2-72868JF669
PE
NBP2-72868PE
PerCP
NBP2-72868PCP
Unconjugated
NBP2-46080
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46080
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Nab2 Antibody (OTI5D7) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 178-442 of human NAB2 (NP_005958) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NAB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
56.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Nab2 Antibody (OTI5D7) - Azide and BSA Free

  • EGR-1-binding protein 2
  • MADEREGR1 binding protein 2
  • Melanoma-associated delayed early response protein
  • MGC75085
  • NGFI-A binding protein 2 (EGR1 binding protein 2)
  • NGFI-A-binding protein 2
  • Protein MADER

Background

Transcriptional control is in part regulated by interactions between DNA-bound transcription factors, such as Egr-1/NGFI-A, and coregulatory proteins, such as NAB (for NGFI-A-binding proteins). The evolutionarily conserved NAB proteins, NAB1 and NAB2, are corepressors of EGF-1/NGFI-A. Both NAB1 and NAB2 contain an amino terminal NAB conserved domain 1 (NCB1), which is required for binding NGFI-A, and a carboxy terminal NCD2 domain, which is responsible for the repressor function of NAB proteins. NAB2 is principally localized in the nucleus and may play a role in the downregulation of NGFI-A activity as well as in controlling fundamental processes such as cell division, differentiation and apoptosis. NAB2 has a predicted molecular mass of 56 kDa and localizes to chromosome 12q13.3-14.1, a region that is rearranged in several solid tumors, lipomas and liposarcomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Nab2 Antibody
(OTI5D7) - Azide and ...
NBP2-72868
Nab2 Antibody (OTI5D7) - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: WB, IHC

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NAB2