Myosin Phosphatase [p Thr850, p Thr853] Antibody Summary
Description |
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
Immunogen |
Synthetic peptide corresponding to aa845-855, C-EKRRS[pT]GVSFW of chicken MYPT1 with an N-terminal cysteine added for conjugation purposes. Phosphorylation site corresponds to Thr853 in human MYPT1. |
Modification |
p Thr853, p Thr850 |
Specificity |
Recognizes recombinant truncated MYPT1 phosphorylated on Thr853, MW ~80kDa |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
PPP1R12A |
Purity |
Protein A purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
|
Application Notes |
Western Blot: Detects MYPT1 by preincubation with ROKa/ROK-II active or C2C12 lysates treated with pervanadate. Optimal dilutions to be determined by the researcher. |
Reactivity Notes
Crossreactivity: human (Thr853, O014974), chicken (Thr850, Q90623), mouse (Thr852, Q9DBR7) and rat (Thr855, Q10728).
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
0.1M Tris-Glycine (pH 7.4), 0.15M NaCl and 30% Glycerol |
Preservative |
0.05% Sodium Azide |
Concentration |
1 mg/ml |
Purity |
Protein A purified |
Notes
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Alternate Names for Myosin Phosphatase [p Thr850, p Thr853] Antibody
Background
Myosin phosphatase target subunit 1 (MYPT1), which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho, as previously shown (6). To understand the role of MYPT1 in the regulation of the cytoskeleton in human diseases, a 4855-bp cDNA was cloned for human MYPT1 using the rat MYPT1 cDNA as probe. Sequencing analysis has revealed that human MYPT1 contains 1030 amino acid residues with a calculated molecular mass of approximately 115kD. Fluorescence in situ hybridization analysis placed the human MYPT1 gene on chromosome 12q15-q21.2. Radiation hybrid mapping has shown that the human MYPT1 gene is located very near the highly polymorphic marker CHLC.GATA65A12, which lies between D12S350 and D12S106.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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