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![Western Blot: Myosin Phosphatase Antibody [NB110-38889] - Detection of human MYPT1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MYPT1 antibody NB110-38889 (lot NB110-38889-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate (B). MYPT1 was also immunoprecipitated by a previous lot (lot NB110-38889-1) of this antibody and by another rabbit anti-MYPT1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Western-Blot-NB110-38889-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Myosin Phosphatase Antibody [NB110-38889] - Detection of human MYPT1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MYPT1 antibody NB110-38889 (lot NB110-38889-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate (B). MYPT1 was also immunoprecipitated by a previous lot (lot NB110-38889-1) of this antibody and by another rabbit anti-MYPT1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-75097] - (4ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nbp2-75097_goat-polyclonal-myosin-phosphatase-antibody-1311202311455812.jpg "Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-75097] - (4ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.")
![Immunocytochemistry/Immunofluorescence: Myosin Phosphatase Antibody [NBP2-75097] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp2-75097_goat-polyclonal-myosin-phosphatase-antibody-13112023114762.jpg)
![Flow Cytometry: Myosin Phosphatase Antibody [NBP2-75097] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-75097_goat-polyclonal-myosin-phosphatase-antibody-1311202311455813.jpg)
![Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-75097] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.](http://images.novusbio.com/fullsize/nbp2-75097_goat-polyclonal-myosin-phosphatase-antibody-1311202311455817.jpg)
![Immunocytochemistry/Immunofluorescence: Myosin Phosphatase Antibody [NBP2-75097] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp2-75097_goat-polyclonal-myosin-phosphatase-antibody-1311202311455810.jpg)
![Western Blot: Myosin Phosphatase Antibody [NBP2-68929] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Western-Blot-NBP2-68929-img0001.jpg "Western Blot: Myosin Phosphatase Antibody [NBP2-68929] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929] - Stainng of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP2-68929-img0006.jpg)
![Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929] - Staining of human liver shows low positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP2-68929-img0003.jpg)
![Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP2-68929-img0004.jpg)
![Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-68929] - Staining of human prostate shows weak to moderate cytoplasmic positivity in glanular and smooth muscle cells.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP2-68929-img0005.jpg)
![Myosin Phosphatase [p Thr853] Antibody [NBP2-13185] - Lane 1: untreated C2C12 lysate| Lane 2: pervanadate treated C2C12 lysate. Resolved by electrophoresis, transferred to nitrocellulose and probed with Myosin Phosphatase [p Thr853] antibody (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Arrow indicates phosphorylated MYPT1 substrate (80kD).](http://images.novusbio.com/fullsize/Myosin-Phosphatase-[p-Thr853]-Antibody-NBP2-13185-img0001.jpg "Myosin Phosphatase [p Thr853] Antibody [NBP2-13185] - Lane 1: untreated C2C12 lysate| Lane 2: pervanadate treated C2C12 lysate. Resolved by electrophoresis, transferred to nitrocellulose and probed with Myosin Phosphatase [p Thr853] antibody (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Arrow indicates phosphorylated MYPT1 substrate (80kD).")
![Myosin Phosphatase [p Thr853] Antibody [NBP2-13185] - Lane 1: ROKa/ROCK-II only Lane 2: MYPT1 substrate only Lane 3: MYPT1 substrate posphorylated by ROKa/ROCK-II 3](http://images.novusbio.com/fullsize/Myosin-Phosphatase-[p-Thr853]-Antibody-NBP2-13185-img0002.jpg)
![Western Blot: Myosin Phosphatase [p Thr853] Antibody (SA19) [NBP2-29680] - Untreated HEK293 cell lysate (lane 1) and HEK293 cell lysate phosphorylated by ROKalpha /ROCKII (lane 2) were probed with anti-phospho-MyPT1 (Thr850), clone SA19 (1:1000 dilution).](http://images.novusbio.com/fullsize/Myosin-Phosphatase-p-Thr853-Antibody-SA19-Western-Blot-NBP2-29680-img0001.jpg "Western Blot: Myosin Phosphatase [p Thr853] Antibody (SA19) [NBP2-29680] - Untreated HEK293 cell lysate (lane 1) and HEK293 cell lysate phosphorylated by ROKalpha /ROCKII (lane 2) were probed with anti-phospho-MyPT1 (Thr850), clone SA19 (1:1000 dilution).")
![Immunocytochemistry/Immunofluorescence: Myosin Phosphatase Antibody [NBP2-58950] - Staining of human cell line U-2 OS shows localization to cytosol & actin filaments.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58950-img0001.jpg "Immunocytochemistry/Immunofluorescence: Myosin Phosphatase Antibody [NBP2-58950] - Staining of human cell line U-2 OS shows localization to cytosol & actin filaments.")
![Western Blot: Myosin Phosphatase Overexpression Lysate (Adult Normal) [NBL1-14674] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Myosin Phosphatase.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14674-img0002.jpg "Western Blot: Myosin Phosphatase Overexpression Lysate (Adult Normal) [NBL1-14674] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Myosin Phosphatase.")
![Western Blot: Myosin Phosphatase Overexpression Lysate (Adult Normal) [NBP2-11066] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Myosin Phosphatase.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11066-img0001.jpg "Western Blot: Myosin Phosphatase Overexpression Lysate (Adult Normal) [NBP2-11066] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Myosin Phosphatase.")
![Western Blot: Myosin Phosphatase Antibody [NBP3-16931] - Western blot analysis of extracts of Jurkat cells, using Myosin Phosphatase Rabbit pAb (NBP3-16931) at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Western-Blot-NBP3-16931-img0001.jpg "Western Blot: Myosin Phosphatase Antibody [NBP3-16931] - Western blot analysis of extracts of Jurkat cells, using Myosin Phosphatase Rabbit pAb (NBP3-16931) at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.")