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Myosin Phosphatase [p Thr853] Antibody (SA19)

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Western Blot: Myosin Phosphatase [p Thr853] Antibody (SA19) [NBP2-29680] - Untreated HEK293 cell lysate (lane 1) and HEK293 cell lysate phosphorylated by ROKalpha /ROCKII (lane 2) were probed with anti-phospho-MyPT1 ...read more

Product Details

Summary
Reactivity Hu, ChSpecies Glossary
Applications WB
Clone
SA19
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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Myosin Phosphatase [p Thr853] Antibody (SA19) Summary

Description
Relevant Entrez Gene Numbers: NM_002480
Additional Information
Recombinant Monoclonal Antibody.
Immunogen
KLH-conjugated, synthetic peptide containing the sequence.RS[pT]GV., in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.
Modification
p Thr853
Specificity
MYPT1 phosphorylated on Thr850
doublet at 130kDa weight may be detected.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PPP1R12A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Publications
Read Publication using
NBP2-29680 in the following applications:

  • WB
    1 publication

Reactivity Notes

Chicken, Human

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
(0.1M Tris-glycine, pH 7.4, 0.15M NaCl, and 30% glycerol
Preservative
No Preservative
Purity
Protein A or G purified

Notes

Relevant Entrez Gene Numbers: NM_002480

Alternate Names for Myosin Phosphatase [p Thr853] Antibody (SA19)

  • MBSmyosin phosphatase, target subunit 1
  • MGC133042
  • Myosin phosphatase target subunit 1
  • Myosin phosphatase-targeting subunit 1
  • MYPT1protein phosphatase 1 regulatory subunit 12A
  • protein phosphatase 1, regulatory (inhibitor) subunit 12A
  • Protein phosphatase myosin-binding subunit

Background

The major protein phosphatase-1 (PP1) is composed of 3 subunits with apparent molecular mass of 110-130, 37 & 20 kDa. The 110-130 kDa component act as a regulatory subunit known as myosine binding (MBS) or myosine phosphatae targeting subunit (MYPT). The N-terminal portion of MYPT binds to both PP1c-delta and phosphorylated myosin, thereby increasing myosin phosphatase activity. Two isoform of MYPT have been identified (MYPT1 & MYPT2) and share 61% sequence identity. While MYPT1 is widely distributed in human tissues, MYPT2 is mostly detected in brain and heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myosin Phosphatase Antibody (NBP2-29680)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol PPP1R12A
Uniprot