Reactivity | Hu, Mu, Rt, ChSpecies Glossary |
Applications | IHC |
Clone | SPM427 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Format | IHC-Prediluted |
Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
Immunogen | Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180-189. (Uniprot: P15172) |
Epitope | aa 180-189 |
Localization | Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation. |
Marker | Rhabdomyosarcoma Marker |
Specificity | Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this monoclonal antibody maps between amino acid 180-189 in the C-terminal of Mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms sarcomas and alveolar soft part sarcomas. |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | MYOD1 |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at 4C. |
Buffer | 10 mM PBS with 0.05% BSA |
Preservative | 0.05% Sodium Azide |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MyoD Antibody (NBP2-44677)Find related products by research area.
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Gene Symbol | MYOD1 |