![Immunocytochemistry/Immunofluorescence: MS4A4A Antibody (5C12) - Chimeric [NBP3-12043] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 5C12 (NBP3-12043) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12043, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/MS4A4A-Antibody-5C12-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12043-img0001.jpg "Immunocytochemistry/Immunofluorescence: MS4A4A Antibody (5C12) - Chimeric [NBP3-12043] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 5C12 (NBP3-12043) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12043, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IP |
| Clone | 5C12 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was generated by immunizing C57BL/6 mice against mouse cell line 300-19 expressing transfected human membrane-spanning 4-domains subfamily A member 4A (MS4A4A)-GFP. |
| Specificity | This antibody is specific for human extracellular MS4A4A. MS4A (membrane-spanning 4-domain family, subfamily A) is a large family of proteins that includes at least 26 members in mouse and humans. Flanked by amino- and carboxyl- cytoplasmic regions, MS4A family members contain four highly conserved transmembrane domains connected by short extracellular and cytoplasmic loops. MS4A4A is likely to be a component of oligomeric cell surface complexes involved in signal transduction in diverse cell lineages. |
| Isotype | IgG Lambda |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MS4A4A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | MS4A4A |
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