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MRLC2 Antibody - BSA Free

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Western Blot: MRLC2 Antibody [NBP3-05574] - Western blot analysis of extracts of various cell lines, using MRLC2 antibody (NBP3-05574) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

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Catalog# & Formulation Size Price
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MRLC2 Antibody - BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human MRLC2 (NP_291024.1). MSSKKAKTKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYL12B
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for MRLC2 Antibody - BSA Free

  • MLC-2
  • MLC20
  • MLC-2A
  • MLC-B
  • MRLC2SHUJUN-1
  • MYLC2B
  • myosin regulatory light chain 12B
  • myosin regulatory light chain 2
  • Myosin regulatory light chain 20 kDa
  • Myosin regulatory light chain 2-B, smooth muscle isoform
  • Myosin regulatory light chain MRLC2
  • myosin, light chain 12B, regulatory

Background

The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain,such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments(Iwasaki et al., 2001 (PubMed 11942626)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYL12B