Novus Biologicals products are now on bio-techne.com

MRLC2 Antibody

Images

 
Western Blot: MRCL3 Antibody [NBP2-54719] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: MRLC2 Antibody [NBP2-54719] - MRCL3 Antibody [NBP2-54719] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli fibrillar center.
Immunohistochemistry-Paraffin: MRCL3 Antibody [NBP2-54719] - Staining of human smooth muscle shows moderate cytoplasmic positivity in smooth muscle cells.
Western Blot: MRCL3 Antibody [NBP2-54719] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251 MG Lane 4: Human plasma Lane 5: Human Liver tissue Lane 6: ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MRLC2 Antibody Summary

Immunogen
This antibody was developed against a Recombinant Protein corresponding to amino acids: YREAPIDKKGNFNYIEFTRILKHGAKDKDD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYL12B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MRLC2 Recombinant Protein Antigen (NBP2-54719PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MRLC2 Antibody

  • MLC-2
  • MLC20
  • MLC-2A
  • MLC-B
  • MRLC2SHUJUN-1
  • MYLC2B
  • myosin regulatory light chain 12B
  • myosin regulatory light chain 2
  • Myosin regulatory light chain 20 kDa
  • Myosin regulatory light chain 2-B, smooth muscle isoform
  • Myosin regulatory light chain MRLC2
  • myosin, light chain 12B, regulatory

Background

The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain,such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments(Iwasaki et al., 2001 (PubMed 11942626)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-85541
Species: Hu, Mu
Applications: IHC, IHC-Fr, IHC-P, WB
NBP2-67486
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-62682
Species: Hu
Applications: IHC, IHC-P
NBP1-81555
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-62625
Species: Hu
Applications: IHC, IHC-P
H00085366-M01
Species: Hu, Mu
Applications: ELISA, KD, WB
NBP1-58359
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-59690
Species: Am, Av, Fi, Ma, Ze
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IP, WB
MAB4470
Species: All-Multi
Applications: ICC, IHC, WB
NBP2-82026
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NB100-91273
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-14871
Species: Mu, Rt
Applications: ICC/IF, WB
NB120-2860
Species: Ba, Bv, Ch, Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-15669
Species: Mu, Rt, Ze
Applications: IHC-WhMt, IHC, WB
NB110-38889
Species: Hu
Applications: IP, WB
NBP2-94899
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
NBP2-14873
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-37897
Species: Hu
Applications: IHC, IHC-P, Simple Western, WB
NB100-624
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-54719
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

Publications for MRLC2 Antibody (NBP2-54719) (0)

There are no publications for MRLC2 Antibody (NBP2-54719).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MRLC2 Antibody (NBP2-54719) (0)

There are no reviews for MRLC2 Antibody (NBP2-54719). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MRLC2 Antibody (NBP2-54719) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MRLC2 Products

Research Areas for MRLC2 Antibody (NBP2-54719)

Find related products by research area.

Blogs on MRLC2

There are no specific blogs for MRLC2, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MRLC2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol MYL12B