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MRLC2 Antibody (SR1293)

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Western Blot: MRLC2 Antibody (SR1293) [NBP3-22501] - Western blot analysis using NBP3-22501 in Jurkat cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
SR1293
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MRLC2 Antibody (SR1293) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human MRLC2 (Uniprot #: O14950)
Specificity
Detects endogenous levels of total MRLC2
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MYL12B
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for MRLC2 Antibody (SR1293)

  • MLC-2
  • MLC20
  • MLC-2A
  • MLC-B
  • MRLC2SHUJUN-1
  • MYLC2B
  • myosin regulatory light chain 12B
  • myosin regulatory light chain 2
  • Myosin regulatory light chain 20 kDa
  • Myosin regulatory light chain 2-B, smooth muscle isoform
  • Myosin regulatory light chain MRLC2
  • myosin, light chain 12B, regulatory

Background

The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain,such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments(Iwasaki et al., 2001 (PubMed 11942626)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MRLC2 Antibody (NBP3-22501) (0)

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Secondary Antibodies

 

Isotype Controls

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Contact Information

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Bioinformatics

Gene Symbol MYL12B