Novus Biologicals products are now on bio-techne.com

MPG Antibody

Images

 
Western Blot: MPG Antibody [NB300-870] - HEK293 lysate (RIPA buffer, 35 ug total protein). Primary antibody at 1 ug/ml incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MPG Antibody [NB300-870] - Human uterus. Antibody at 5 ug/ml. Antigen retrieval with citrate buffer at pH 6. AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

MPG Antibody Summary

Immunogen
Peptide with sequence C-SVVDRVAEQDTQA corresponding to C-Terminus according to NP_002425.2, NP_001015052.1, NP_001015054.1.
Specificity
This antibody is expected to recognize all three reported isoforms (NP_002425.2, NP_001015052.1 and NP_001015054.1)
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MPG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in lysates of HEK293 and K562 (calculated MW of 32.9 kDa band according to NP_002425.2).
Control
K-562 Whole Cell Lysate
MPG Overexpression Lysate
Publications
Read Publication using NB300-870.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MPG Antibody

  • 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on16p
  • 3-alkyladenine DNA glycosylase
  • 3-methyladenine DNA glycosidase
  • AAG
  • ADPG
  • anpg
  • APNG
  • CRA36.1 (3-methyl-adenine DNA glycosylase)
  • CRA36.1
  • DNA-3-methyladenine glycosylase
  • EC 3.2.2.21
  • MDGalkyladenine DNA glycosylase
  • Mid1
  • N-methylpurine-DNA glycosylase, MPG
  • N-methylpurine-DNA glycosylasePIG16
  • PIG11
  • proliferation-inducing protein 11
  • proliferation-inducing protein 16

Background

MPG (N-methylpurine DNA glycosylase, or 3-methyladenine (3MeA) DNA glycosylase) repairs the 3MeA lethal lesion blocking DNA replication in Escherichia coli. Analysis of human cDNA libraries found a cDNA that would hybridize to human genomic DNA but not to E. coli or yeast DNA. This MPG gene has been mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
NB100-116
Species: Hu, Mu, Pm, Rt
Applications: ChIP, ELISA, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, PLA, Simple Western, WB
NB100-168
Species: Hu, Mu(-)
Applications: CyTOF-ready, Flow-IC, Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NBP2-58917
Species: Hu
Applications: IHC, IHC-P, WB
NB100-858
Species: Gp, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, PEP-ELISA, WB
NB300-605
Species: Hu, Mu, Po, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-16182
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-13075
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
H00008086-M02
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
H00005018-D01P
Species: Hu
Applications: ICC/IF, WB
NBP1-86616
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-88866
Species: Hu
Applications: IHC, IHC-P
NB100-106
Species: Hu, Mu, Po, Pm, Rb, Rt
Applications: ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, WB
NBP3-16735
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB300-870
Species: Hu
Applications: WB, ELISA, IHC

Publications for MPG Antibody (NB300-870)(1)

Reviews for MPG Antibody (NB300-870) (0)

There are no reviews for MPG Antibody (NB300-870). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MPG Antibody (NB300-870) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional MPG Products

Research Areas for MPG Antibody (NB300-870)

Find related products by research area.

Blogs on MPG

There are no specific blogs for MPG, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MPG Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol MPG
Entrez
Uniprot