Home » MOG » MOG Lysates » MOG Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

MOG Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: MOG Overexpression Lysate [NBP2-04536] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MOG.
Western Blot: MOG Overexpression Lysate [NBP2-04536] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MOG.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals offers 100% Guarantee

MOG Overexpression Lysate Summary

Description

MOG1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC222455

Accession#: NM_206809

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
MOG

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
25.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for MOG Overexpression Lysate

  • MGC26137
  • MOG Ig-AluB
  • MOG
  • MOGIG2
  • myelin oligodendrocyte glycoprotein
  • myelin-oligodendrocyte glycoprotein

Background

The product of this gene is a membrane protein expressed on the oligodendrocyte cell surface and the outermost surface of myelin sheaths. Due to this localization, it is a primary target antigen involved in immune-mediated demyelination. This protein may be involved in completion and maintenance of the myelin sheath and in cell-cell communication. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAG/Siglec-4a
538-MG
MAG/Siglec-4a
(19 Publications)
Species: Rt
Applications: BA
MBP Antibody (12)
- BSA Free
NB600-717
Immunohistochemistry-Frozen MBP Antibody (12) - BSA Free
Immunohistochemistry-Frozen MBP Antibody (12) - BSA Free
Immunocytochemistry/ Immunofluorescence MBP Antibody (12) - BSA Free
(3 Reviews)
(46 Publications)
Species: Bv
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RIA, RI, WB
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(459 Publications)
Species: Hu
Applications: BA
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(52 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
MBL Antibody
AF2307
MBL Antibody
(9 Publications)
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), WB
MBP-1 Antibody -
Azide and BSA Free
NBP3-04784
Western Blot MBP-1 Antibody - Azide and BSA Free
Immunohistochemistry-Paraffin MBP-1 Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence MBP-1 Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(824 Publications)
Species: Hu
Applications: BA
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(307 Publications)
Species: Mu
Applications: ELISA
Myelin PLP
Antibody - BSA Free
NBP1-87781
Immunohistochemistry-Paraffin Myelin PLP Antibody - BSA Free
Immunohistochemistry-Paraffin Myelin PLP Antibody - BSA Free
Immunohistochemistry-Paraffin Myelin PLP Antibody - BSA Free
(1 Review)
(6 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(676 Publications)
Species: Mu
Applications: ELISA
Peroxiredoxin 5
Antibody [Unconjugate
AF5724
Western Blot Peroxiredoxin 5 Antibody [Unconjugated]
Immunocytochemistry Peroxiredoxin 5 Antibody [Unconjugated]
Immunocytochemistry Peroxiredoxin 5 Antibody [Unconjugated]
Species: Hu, Mu, Rt
Applications: ICC, WB
PDXP Antibody -
Azide and BSA Free
NBP2-93574
Western Blot PDXP Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence PDXP Antibody - Azide and BSA Free
Immunohistochemistry-Paraffin PDXP Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
PTHLH/PTHrP
Antibody (677939) [Un ...
MAB6734
Immunohistochemistry PTHLH/PTHrP Antibody (677939) [Unconjugated]
(2 Publications)
Species: Hu
Applications: IHC
IL-17/IL-17A
[Biotin]
DY421
N/A IL-17/IL-17A [Biotin]
(193 Publications)
Species: Mu
Applications: ELISA
OMgp Antibody
AF1674
OMgp Antibody
(5 Publications)
Species: Mu
Applications: WB
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(379 Publications)
Species: Hu
Applications: BA
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
FoxP3 Antibody -
BSA Free
NB100-39002
Flow Cytometry FoxP3 Antibody - BSA Free
Flow Cytometry FoxP3 Antibody - BSA Free
Immunohistochemistry-Paraffin FoxP3 Antibody - BSA Free
(2 Reviews)
(66 Publications)
Species: Bv, Hu, Mu, Rt
Applications: Dual ISH-IHC, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
CD8 Antibody
(53-6.7) - BSA Free
NBP1-49045
Immunohistochemistry-Paraffin CD8 Antibody (53-6.7) - BSA Free
Immunocytochemistry/ Immunofluorescence CD8 Antibody (53-6.7) - BSA Free
Immunohistochemistry-Frozen CD8 Antibody (53-6.7) - BSA Free
(3 Reviews)
(28 Publications)
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc

Publications for MOG Lysate (NBP2-04536) (0)

There are no publications for MOG Lysate (NBP2-04536).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MOG Lysate (NBP2-04536) (0)

There are no reviews for MOG Lysate (NBP2-04536). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MOG Lysate (NBP2-04536) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional MOG Products

Blogs on MOG

There are no specific blogs for MOG, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MOG Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol MOG
Uniprot