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Moesin Antibody (MSN/491) [PE/Cy5.5]

 
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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, CyTOF-ready
Clone
MSN/491
Clonality
Monoclonal
Host
Mouse
Conjugate
PE/Cy5.5

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34685AF350
Alexa Fluor 405
NBP2-34685AF405
Alexa Fluor 488
NBP2-34685AF488
Alexa Fluor 532
NBP2-34685AF532
Alexa Fluor 594
NBP2-34685AF594
Alexa Fluor 647
NBP2-34685AF647
Alexa Fluor 700
NBP2-34685AF700
Alexa Fluor 750
NBP2-34685AF750
Allophycocyanin
NBP2-34685APC
Allophycocyanin/Cy7
NBP2-34685APCCY7
Biotin
NBP2-34685B
CoraFluor 1
NBP2-34685CL1
DyLight 350
NBP2-34685UV
DyLight 405
NBP2-34685V
DyLight 488
NBP2-34685G
DyLight 550
NBP2-34685R
DyLight 594
NBP2-34685DL594
DyLight 650
NBP2-34685C
DyLight 680
NBP2-34685FR
DyLight 755
NBP2-34685IR
FITC
NBP2-34685F
HRP
NBP2-34685H
Janelia Fluor 525
NBP2-34685JF525
Janelia Fluor 549
NBP2-34685JF549
Janelia Fluor 646
NBP2-34685JF646
Janelia Fluor 669
NBP2-34685JF669
PE
NBP2-34685PE
PE/Atto594
NBP2-34685PEATT594
PE/Cy7
NBP2-34685PECY7
PerCP
NBP2-34685PCP
Unconjugated
NBP2-32875
mFluor Violet 450 SE
NBP2-34685MFV450
mFluor Violet 500 SE
NBP2-34685MFV500
mFluor Violet 610 SE
NBP2-34685MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-32875
Azide and BSA Free
NBP2-34685-0.1mg
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Moesin Antibody (MSN/491) [PE/Cy5.5] Summary

Immunogen
Recombinant full-length human Moesin protein (Uniprot: P26038)
Localization
Cytoplasmic & Cell Surface
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MSN
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Moesin Antibody (MSN/491) [PE/Cy5.5]

  • Membrane-organizing extension spike protein
  • moesin

Background

Moesin (membrane-organizing extension spike protein) has previously been characterized as a possible receptor protein for heparan sulfate and also as a cytoskeletal linker protein that stabilizes cell surface microvilli, filopodia and lamellipodia. Data indicate that moesin is identical to the 77-kDa band that copurifies with ezrin in its isolation from human placenta (1). Members of the ezrin-radixin-moesin (ERM) family of membrane-cytoskeletal linking proteins have NH2- and COOH-terminal domains that associate with the plasma membrane and the actin cytoskeleton, respectively (2). It has been demonstrated that ezrin-radixin-moesin proteins are rapidly inactivated after antigen recognition through a Vav1-Rac1 pathway. The resulting disanchoring of the cortical actin cytoskeleton from the plasma membrane decreased cellular rigidity, leading to more efficient T cell-antigen-presenting cell conjugate formation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Moesin Antibody (NBP2-34685PECY55). (Showing 1 - 1 of 1 FAQ).

  1. I am looking to use shRNA to inhibit Moesin expression. I have had people advise me that my initial MOI should be low as 'less is more' and 'a little goes a long way' in terms of siRNA. I was wondering if you could elaborate on this for me and explain why my initial MOI should be low.
    • The reason for a low MOI is most likely because RNAi is a very strong and efficient technique. Wikipedia does a good job of explaining <a href="http://en.wikipedia.org/wiki/RNA_interference" target="_blank">RNA interference</a>. However, I would imagine that in a cell, there will be at most 1-2 copies of the gene mRNA present at any given time, unless you're dealing with a highly expressed protein such as Actin, where I would imagine silencing Actin would be lethal to the cell. I can imagine a few reasons to not use too much siRNA. First, it is expensive, so you don't want to waste it. Second, using too much would cause there to be a lot of non-translatable RNA present in the cell, which could trigger an immune response, as the presence of uncapped RNAs can indicate presence of a virus and one of the TLRs may respond to this.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MSN