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Moesin Antibody (MSN/491) [Janelia Fluor® 669]

 
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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, CyTOF-ready
Clone
MSN/491
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34685AF350
Alexa Fluor 405
NBP2-34685AF405
Alexa Fluor 488
NBP2-34685AF488
Alexa Fluor 532
NBP2-34685AF532
Alexa Fluor 594
NBP2-34685AF594
Alexa Fluor 647
NBP2-34685AF647
Alexa Fluor 700
NBP2-34685AF700
Alexa Fluor 750
NBP2-34685AF750
Allophycocyanin
NBP2-34685APC
Allophycocyanin/Cy7
NBP2-34685APCCY7
Biotin
NBP2-34685B
CoraFluor 1
NBP2-34685CL1
DyLight 350
NBP2-34685UV
DyLight 405
NBP2-34685V
DyLight 488
NBP2-34685G
DyLight 550
NBP2-34685R
DyLight 594
NBP2-34685DL594
DyLight 650
NBP2-34685C
DyLight 680
NBP2-34685FR
DyLight 755
NBP2-34685IR
FITC
NBP2-34685F
HRP
NBP2-34685H
Janelia Fluor 525
NBP2-34685JF525
Janelia Fluor 549
NBP2-34685JF549
Janelia Fluor 646
NBP2-34685JF646
PE
NBP2-34685PE
PE/Atto594
NBP2-34685PEATT594
PE/Cy5.5
NBP2-34685PECY55
PE/Cy7
NBP2-34685PECY7
PerCP
NBP2-34685PCP
Unconjugated
NBP2-32875
mFluor Violet 450 SE
NBP2-34685MFV450
mFluor Violet 500 SE
NBP2-34685MFV500
mFluor Violet 610 SE
NBP2-34685MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-32875
Azide and BSA Free
NBP2-34685-0.1mg
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Moesin Antibody (MSN/491) [Janelia Fluor® 669] Summary

Immunogen
Recombinant full-length human Moesin protein (Uniprot: P26038)
Localization
Cytoplasmic & Cell Surface
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MSN
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Moesin Antibody (MSN/491) [Janelia Fluor® 669]

  • Membrane-organizing extension spike protein
  • moesin

Background

Moesin (membrane-organizing extension spike protein) has previously been characterized as a possible receptor protein for heparan sulfate and also as a cytoskeletal linker protein that stabilizes cell surface microvilli, filopodia and lamellipodia. Data indicate that moesin is identical to the 77-kDa band that copurifies with ezrin in its isolation from human placenta (1). Members of the ezrin-radixin-moesin (ERM) family of membrane-cytoskeletal linking proteins have NH2- and COOH-terminal domains that associate with the plasma membrane and the actin cytoskeleton, respectively (2). It has been demonstrated that ezrin-radixin-moesin proteins are rapidly inactivated after antigen recognition through a Vav1-Rac1 pathway. The resulting disanchoring of the cortical actin cytoskeleton from the plasma membrane decreased cellular rigidity, leading to more efficient T cell-antigen-presenting cell conjugate formation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Moesin Antibody (NBP2-34685JF669). (Showing 1 - 1 of 1 FAQ).

  1. I am looking to use shRNA to inhibit Moesin expression. I have had people advise me that my initial MOI should be low as 'less is more' and 'a little goes a long way' in terms of siRNA. I was wondering if you could elaborate on this for me and explain why my initial MOI should be low.
    • The reason for a low MOI is most likely because RNAi is a very strong and efficient technique. Wikipedia does a good job of explaining <a href="http://en.wikipedia.org/wiki/RNA_interference" target="_blank">RNA interference</a>. However, I would imagine that in a cell, there will be at most 1-2 copies of the gene mRNA present at any given time, unless you're dealing with a highly expressed protein such as Actin, where I would imagine silencing Actin would be lethal to the cell. I can imagine a few reasons to not use too much siRNA. First, it is expensive, so you don't want to waste it. Second, using too much would cause there to be a lot of non-translatable RNA present in the cell, which could trigger an immune response, as the presence of uncapped RNAs can indicate presence of a virus and one of the TLRs may respond to this.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MSN