MNX1/HLXB9 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

The homeobox gene Hb9/Hlxb9, is expressed selectively by motor neurons (MNs) in the developing vertebrate CNS. Hb9, which encodes a factor central to MN development regulates neuronal fate by restricting expression of Lim3 and Even-skipped (Eve), two homeodomain (HD) proteins required for development of distinct neuronal classes. In the vertebrate CNS, HB9/MNR2 transcription factors are specifically expressed in all somatic motoneurons and are necessary to distinguish motoneurons from interneurons. Hb9 is also a transcription factor expressed in lymphoid and pancreatic tissue. In humans, HB9 mutation causes an autosomal dominant form of sacral agenesis.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBL1-13167

• MNX1/HLXB9 Antibodies
• MNX1/HLXB9 Lysate
• MNX1/HLXB9 Protein