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MMP-8 Antibody (100619) [Unconjugated]

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MMP-8 expression in; primary normal and DCIS myoepithelial cells and a cell line model. a Normal and ductal carcinoma in situ (DCIS) tissue immunohistochemically stained for matrix metalloproteinase-8 (MMP-8) (Atlas, HPA02122, 1:1000). Clear MMP-8 staining can be observed in the normal myoepithelial cells (MECs), which is absent in the DCIS section. Magnification × 400. b Normal MECs and luminal epithelial cells (LECs) were isolated from reduction mammoplasty tissue and DCIS MEC and DCIS LEC were isolated from a patient with high-grade DCIS. Tissues were enzymatically digested and magnetic bead separation was used to isolated specific populations (MEC - ITGB4, LEC - EpCAM). RNA was extracted from the isolated cells and G361 cells (a positive control for MMP-8), converted to cDNA by reverse transcription and then subjected to nested PCR for MMP-8 or PCR of housekeeping gene 18S. The MMP-8 PCR produces a product of 150 bp in normal MEC and G361 lanes, and other lanes only give a weak band. 18S gives a size of 52 bp in all sample lanes. The water (H2O) control lane shows no band. c (i) RNA was extracted from cell lines; G361, N-1089 and beta 6-1089 and converted to cDNA as above. The cDNA was subjected to nested PCR for MMP-8 and bands were identified in G361 and N-1089 but only a weak band was observed in the beta 6-1089 lane. All samples demonstrated a band for 18S. (ii) RIPA protein lysates were produced from N-1089 and beta 6-1089 cells and 20 ug of protein was run on 8% PAGE then transferred to nitrocellulose and probed for MMP-8 (R&D Systems, mAb 908) alpha v beta 6 (Santa Cruz Biotechnology, SC-6632) or HSC70 as a loading control. The gel indicates that N-1089 expresses higher levels of MMP-8 and much lower levels of alpha v beta 6. While beta 6-1089 exhibit much higher levels of alpha v beta 6 and lower levels of MMP-8. (iii) Densitometry of western blots indicates that MMP-8 expression in N-1089 is 2.5-fold higher than N-1089 and (iv) alpha v beta 6 expression in beta 6-1089 is 10-fold higher than N-1089 Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-017-0822-9), licensed under a CC-BY license. Not internally tested by R&D Systems.
MMP-8 expression in; primary normal and DCIS myoepithelial cells and a cell line model. a Normal and ductal carcinoma in situ (DCIS) tissue immunohistochemically stained for matrix metalloproteinase-8 (MMP-8) (Atlas, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP, ELISA(Cap)
Clone
100619
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB908-SP
Carrier Free
MAB908-500
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MMP-8 Antibody (100619) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑8
Phe21-Gly467
Accession # AAZ38714
Specificity
Detects the pro and active forms of human MMP-8 in Western blots. Does not cross-react with recombinant human (rh) MMP‑1, -2, -3, -7, -9,
-10, -12, or -13.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MMP8
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human MMP-8 Biotinylated Antibody (Catalog number BAF908)
Standard: Recombinant Human MMP-8 (Catalog number 908-MP)
Publications
Read Publications using
MAB908 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-8 Antibody (100619) [Unconjugated]

  • CLG1HNC
  • Collagenase 2
  • EC 3.4.24
  • EC 3.4.24.34
  • matrix metallopeptidase 8 (neutrophil collagenase)
  • matrix metalloproteinase 8 (neutrophil collagenase)
  • Matrix metalloproteinase-8
  • MMP8
  • MMP-8
  • neutrophil collagenase
  • PMNL collagenase
  • PMNL-CL

Background

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP‑8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP‑8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP‑8 is capable of cleaving types I, II and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta -casein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis,
MMP‑8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP‑8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexin-like domain. MMP‑8 is heavily glycosylated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-8 Antibody (MAB908)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Activity Assay, ELISA Capture, Western Blot.


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(1)
ELISA Capture
(1)
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(3)
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Bioinformatics

Gene Symbol MMP8
Uniprot