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MMP-2 Antibody [Biotin]

 (3 Publications)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF902
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF902
Carrier Free
AF902
Carrier Free
AF902-SP
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MMP-2 Antibody [Biotin] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP‑2 (R&D Systems, Catalog # 902-MP)
Ile34-Cys660
Accession # P08253
Specificity
Detects pro/active human MMP-2 in Western blots. In this format, approximately 50% cross-reactivity with recombinant mouse MMP‑2 is observed and less than 1% cross-reactivity with recombinant human (rh) MMP-1, rhMMP-3, rhMMP-8, rhMMP-9, rhMMP-10, rhMMP-12, and rhMMP-13 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MMP2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Readout System
Publications
Read Publications using
BAF902 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-2 Antibody [Biotin]

  • 72 kDa gelatinase
  • CLG4
  • CLG4A72 kDa type IV collagenase
  • collagenase type IV-A
  • EC 3.4.24
  • EC 3.4.24.24
  • Gelatinase A
  • matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
  • matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase)
  • Matrix metalloproteinase-2
  • matrix metalloproteinase-II
  • MMP2
  • MMP-2
  • MMP-II
  • MONA
  • neutrophil gelatinase
  • TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-2 Antibody (BAF902)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ELISA Development, ELISA Microarray Development.


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ELISA Development
(1)
ELISA Microarray Development
(1)
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(2)
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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MMP2
Uniprot