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Mind Bomb 1/MIB1 Antibody - BSA Free

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Western Blot: Mind Bomb 1/MIB1 Antibody [NBP2-81754] - Analysis of Mind Bomb 1/MIB1in A431 cell lysate with Mind Bomb 1/MIB1 antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: Mind Bomb 1/MIB1 Antibody [NBP2-81754] - Immunocytochemistry of Mind Bomb 1/MIB1 in A431 cells with Mind Bomb 1/MIB1 antibody at 5 ug/mL.
Immunocytochemistry/ Immunofluorescence: Mind Bomb 1/MIB1 Antibody - BSA Free [NBP2-81754] - Immunofluorescence of Mind Bomb 1/MIB1 in A431 cells with Mind Bomb 1/MIB1 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Mind Bomb 1/MIB1 Antibody - BSA Free Summary

Immunogen
MIB1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human MIB1. The immunogen is located within amino acids 920 - 970 of MIB1. Amino Acid Squence: IPVLQKDKDNTNVNADVQK
Specificity
Mind Bomb 1/MIB1 antibody is human, mouse and rat reactive. At least three isoforms of Mind Bomb 1/MIB1 are known to exist.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MIB1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 5-20 ug/ml
  • Western Blot 1-2 ug/ml
Theoretical MW
103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for Mind Bomb 1/MIB1 Antibody - BSA Free

  • DAPK-interacting protein 1
  • DIP1
  • DIP-1FLJ90676
  • DKFZp686I0769
  • DKFZp761M1710
  • E3 ubiquitin-protein ligase MIB1
  • EC 6.3.2
  • EC 6.3.2.-
  • KIAA1323
  • MGC129660
  • MIB
  • MIB1
  • Mind Bomb 1
  • Mind bomb homolog 1
  • mindbomb homolog 1 (Drosophila)
  • ubiquitin ligase mind bomb
  • ubiquitin ligase protein MIB1
  • Zinc finger ZZ type with ankyrin repeat domain protein 2
  • ZZANK2
  • ZZANK2MGC129659
  • ZZZ6

Background

E3 ubiquitin ligase protein mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. It positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. It probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MIB1