![Western Blot: Microsomal Glutathione S-transferase 1 Antibody (6A0S5) [NBP3-15316] - Western blot analysis of extracts of various cell lines, using Microsomal Glutathione S-transferase 1 antibody (NBP3-15316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Microsomal-Glutathione-S-transferase-1-Antibody-6A0S5-Western-Blot-NBP3-15316-img0006.jpg "Western Blot: Microsomal Glutathione S-transferase 1 Antibody (6A0S5) [NBP3-15316] - Western blot analysis of extracts of various cell lines, using Microsomal Glutathione S-transferase 1 antibody (NBP3-15316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 6A0S5 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Microsomal Glutathione S-transferase 1 (P10620). MVDLTQVMDDEVFMAFASYATIILSKMMLMSTATAFYRLTRKVFANPEDCVAFGKGENAKKYLRTDDRVERVRRAHLNDLENIIPFLGIGLLYSLSGPDP |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MGST1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 18 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 0.05% BSA, 50% glycerol, pH7.3 |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Microsomal Glutathione S-transferase 1 Antibody (NBP3-15316)Find related products by research area.
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| Gene Symbol | MGST1 |
![Western Blot: Microsomal Glutathione S-transferase 1 Antibody (6A0S5) [NBP3-15316] - Western blot analysis of extracts of HepG2 cells, using Microsomal Glutathione S-transferase 1 antibody (NBP3-15316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Microsomal-Glutathione-S-transferase-1-Antibody-6A0S5-Western-Blot-NBP3-15316-img0007.jpg "Western Blot: Microsomal Glutathione S-transferase 1 Antibody (6A0S5) [NBP3-15316] - Western blot analysis of extracts of HepG2 cells, using Microsomal Glutathione S-transferase 1 antibody (NBP3-15316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Microsomal Glutathione S-transferase 1 Antibody (6A0S5) [NBP3-15316] -Human liver cancer tissue using MGST1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15316_rabbit-microsomal-glutathione-s-transferase-1-mab-6a0s5-immunohistochemistry-paraffin-211020248747..jpg "Immunohistochemistry-Paraffin: Microsomal Glutathione S-transferase 1 Antibody (6A0S5) [NBP3-15316] -Human liver cancer tissue using MGST1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry-Paraffin: Microsomal Glutathione S-transferase 1 Antibody (6A0S5) [NBP3-15316] - Human liver tissue using MGST1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15316_rabbit-microsomal-glutathione-s-transferase-1-mab-6a0s5-immunohistochemistry-paraffin-211020248844..jpg "Immunohistochemistry-Paraffin: Microsomal Glutathione S-transferase 1 Antibody (6A0S5) [NBP3-15316] - Human liver tissue using MGST1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry-Paraffin: Microsomal Glutathione S-transferase 1 Antibody (6A0S5) [NBP3-15316] -Human thyroid cancer tissue using MGST1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15316_rabbit-microsomal-glutathione-s-transferase-1-mab-6a0s5-immunohistochemistry-paraffin-211020248943..jpg "Immunohistochemistry-Paraffin: Microsomal Glutathione S-transferase 1 Antibody (6A0S5) [NBP3-15316] -Human thyroid cancer tissue using MGST1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")