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Melatonin R1A/MT1/MTNR1A Overexpression Lysate

Images

 
Western Blot: Melatonin Receptor 1A Overexpression Lysate (Adult Normal) [NBL1-13378] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Melatonin Receptor 1A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Melatonin R1A/MT1/MTNR1A Overexpression Lysate Summary

Description

Melatonin R1A/MT1/MTNR1A Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC210385

Accession#: NM_005958

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
MTNR1A

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
39.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for Melatonin R1A/MT1/MTNR1A Overexpression Lysate

  • Mel1a receptor
  • Mel-1A-R
  • Melatonin R1A
  • melatonin receptor 1A
  • melatonin receptor type 1A
  • MelatoninR1A
  • MT1
  • MTNR1A

Background

Melatonin Receptor 1A encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Melatonin R1A/MT1/MTNR1A Lysate (NBL1-13378) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Melatonin R1A/MT1/MTNR1A Lysate (NBL1-13378). (Showing 1 - 2 of 2 FAQ).

  1. I saw that you have the antibodies for the both receptors of melatonin but not listed with FACS application. I need more information about what is better for my assay.
    • At this time, none of our melatonin receptor antibodies have been tested and validated in Flow. Should you choose to purchase and test one of them, you would qualify for the Innovator's Rewards program. You should take a look at each individual antibody, the clonality, conjugates, immunogen region and all relevant data in order to decide which product would be most suitable for your needs.
  2. Do you have MTNR1A polyclonal antibody ?
    • Here are our MTNR1A antibodies that are polyclonal

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Bioinformatics

Gene Symbol MTNR1A