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MEK2 Antibody (OTI8G6) [Janelia Fluor® 525]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
OTI8G6
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 525

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72646AF350
Alexa Fluor 405
NBP2-72646AF405
Alexa Fluor 488
NBP2-72646AF488
Alexa Fluor 532
NBP2-72646AF532
Alexa Fluor 594
NBP2-72646AF594
Alexa Fluor 647
NBP2-72646AF647
Alexa Fluor 700
NBP2-72646AF700
Alexa Fluor 750
NBP2-72646AF750
Allophycocyanin
NBP2-72646APC
Allophycocyanin/Cy7
NBP2-72646APCCY7
Biotin
NBP2-72646B
CoraFluor 1
NBP2-72646CL1
DyLight 350
NBP2-72646UV
DyLight 405
NBP2-72646V
DyLight 488
NBP2-72646G
DyLight 550
NBP2-72646R
DyLight 594
NBP2-72646DL594
DyLight 650
NBP2-72646C
DyLight 680
NBP2-72646FR
DyLight 755
NBP2-72646IR
FITC
NBP2-72646F
HRP
NBP2-72646H
Janelia Fluor 549
NBP2-72646JF549
Janelia Fluor 585
NBP2-72646JF585
Janelia Fluor 635
NBP2-72646JF635
Janelia Fluor 646
NBP2-72646JF646
Janelia Fluor 669
NBP2-72646JF669
PE
NBP2-72646PE
PE/Atto594
NBP2-72646PEATT594
PE/Cy5.5
NBP2-72646PECY55
PE/Cy7
NBP2-72646PECY7
PerCP
NBP2-72646PCP
Unconjugated
NBP1-47836
mFluor Violet 450 SE
NBP2-72646MFV450
mFluor Violet 500 SE
NBP2-72646MFV500
mFluor Violet 610 SE
NBP2-72646MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-47836
Azide and BSA Free
NBP2-72646
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MEK2 Antibody (OTI8G6) [Janelia Fluor® 525] Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human MAP2K2 expression vector
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAP2K2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MEK2 Antibody (OTI8G6) [Janelia Fluor® 525]

  • ERK activator kinase 2
  • MAP2K2
  • MAPKK 2
  • MEK 2
  • MEK2
  • MEK2EC 2.7.12.2
  • mitogen-activated protein kinase kinase 2
  • MKK2
  • MKK2FLJ26075
  • p45
  • PRKMK2MAPKK2

Background

MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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(8 Publications)
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NBP2-72646JF525
MEK2 Antibody (OTI8G6) [Janelia Fluor® 525]
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, CyTOF-ready

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MAP2K2