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ME1 Antibody (736)

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Genetic Strategies: Western Blot: ME1 Antibody (736) [NBP2-43709] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 ...read more
Immunocytochemistry/ Immunofluorescence: ME1 Antibody (736) [NBP2-43709] - HeLa cells were fixed in ice-cold MeOH for 5 min.Green: ME1 protein stained by ME1 antibody [736] diluted at 1:400. Blue: Hoechst 33342 staining.
Western Blot: ME1 Antibody (736) [NBP2-43709] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody [736] diluted at a dilution of 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ShSpecies Glossary
Applications WB, ICC/IF
Clone
736
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

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ME1 Antibody (736) Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ME1. The exact sequence is proprietary.
Predicted Species
Mouse (91%), Rat (91%), Sheep (92%), Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ME1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for ME1 Antibody (736)

  • EC 1.1.1
  • EC 1.1.1.40
  • HUMNDME
  • malate dehydrogenase
  • Malic enzyme 1
  • malic enzyme 1, NADP(+)-dependent, cytosolic
  • malic enzyme 1, soluble
  • Malic enzyme, cytoplasmic
  • MES
  • NADP-dependent malic enzyme
  • NADP-ME
  • pyruvic-malic carboxylase

Background

ME1 encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ME1 Antibody (NBP2-43709) (0)

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mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol ME1