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MCCC2 Antibody

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Immunocytochemistry/ Immunofluorescence: MCCC2 Antibody [NBP2-97284] - Immunofluorescence staining of MCCC2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% ...read more
Immunohistochemistry-Paraffin: MCCC2 Antibody [NBP2-97284] - Immunochemical staining of human MCCC2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MCCC2 Antibody Summary

Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Immunogen
Produced in rabbits immunized with E. coli-derived Human MCCC2 fragment.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCCC2
Purity
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Proclin 300
Purity
Antigen and protein A Affinity-purified

Alternate Names for MCCC2 Antibody

  • 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta
  • MCCase subunit beta
  • MCCBEC 6.4.1.4
  • methylcrotonoyl-CoA carboxylase 2 (beta)
  • methylcrotonoyl-Coenzyme A carboxylase 2 (beta)
  • mitochondrial
  • non-biotin containing subunit of 3-methylcrotonyl-CoA carboxylase

Background

MCCC2 encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MCCC2