![Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01] - Analysis of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody (M01), clone 3B4-G3.Lane 1: GPR3 transfected lysate(35 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GPR3-Antibody-3B4-G3-Western-Blot-H00002827-M01-img0011.jpg "Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01] - Analysis of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody (M01), clone 3B4-G3.Lane 1: GPR3 transfected lysate(35 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, KD |
| Clone | 3B4-G3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | GPR3 (AAH32702, 1 a.a. ~ 330 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MMWGAGSPLAWLSAGSGNVNVSSVGPAEGPTGPAAPLPSPKAWDVVLCISGTLVSCENALVVAIIVGTPAFRAPMFLLVGSLAVADLLAGLGLVLHFAAVFCIGSAEMSLVLVGVLAMAFTASIGSLLAITVDRYLSLYNALTYYSETTVTRTYVMLALVWGGALGLGLLPVLAWNCLDGLTTCGVVYPLSKNHLVVLAIAFFMVFGIMLQLYAQICRIVCRHAQQIALQRHLLPASHYVATRKGIATLAVVLGAFAACWLPFTVYCLLGDAHSPPLYTYLTLLPATYNSMINPIIYAFRNQDVQKVLWAVCCCCSSSKIPFRSRSPSDV |
| Specificity | GPR3 - G protein-coupled receptor 3 |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GPR3 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for GPR3 Antibody (H00002827-M01)Find related products by research area.
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![Immunohistochemistry-Paraffin: GPR3 Antibody (3B4-G3) [H00002827-M01] - Analysis of monoclonal antibody to GPR3 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/GPR3-Antibody-3B4-G3-Immunohistochemistry-Paraffin-H00002827-M01-img0007.jpg "Immunohistochemistry-Paraffin: GPR3 Antibody (3B4-G3) [H00002827-M01] - Analysis of monoclonal antibody to GPR3 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. Antibody concentration 1 ug/ml.")
![Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01] - Analysis of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi ( Cat # H00002827-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody (M01), clone 3B4-G3 (Cat # H00002827-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/GPR3-Antibody-3B4-G3-Western-Blot-H00002827-M01-img0008.jpg "Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01] - Analysis of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi ( Cat # H00002827-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody (M01), clone 3B4-G3 (Cat # H00002827-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01] - GPR3 monoclonal antibody (M01), clone 3B4-G3 Analysis of GPR3 expression in Jurkat.](http://images.novusbio.com/fullsize/GPR3-Antibody-3B4-G3-Western-Blot-H00002827-M01-img0010.jpg "Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01] - GPR3 monoclonal antibody (M01), clone 3B4-G3 Analysis of GPR3 expression in Jurkat.")
![Sandwich ELISA: GPR3 Antibody (3B4-G3) [H00002827-M01] - Detection limit for recombinant GST tagged GPR3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GPR3-Antibody-3B4-G3-Sandwich-ELISA-H00002827-M01-img0009.jpg "Sandwich ELISA: GPR3 Antibody (3B4-G3) [H00002827-M01] - Detection limit for recombinant GST tagged GPR3 is approximately 0.03ng/ml as a capture antibody.")
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