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MCCC2 Antibody

Images

 
Western Blot: MCCC2 Antibody [NBP2-38403] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: MCCC2 Antibody [NBP2-38403] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunohistochemistry-Paraffin: MCCC2 Antibody [NBP2-38403] - Staining of human skeletal muscle using Anti-MCCC2 antibody NBP2-38403.
Western Blot: MCCC2 Antibody [NBP2-38403] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: RT-4Lane 3: U-251 MG
Immunohistochemistry-Paraffin: MCCC2 Antibody [NBP2-38403] - Staining of human skeletal muscle shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: MCCC2 Antibody [NBP2-38403] - Staining in human kidney and skeletal muscle tissues using anti-MCCC2 antibody. Corresponding MCCC2 RNA-seq data are presented ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: MCCC2 Antibody [NBP2-38403] - Staining of human kidney, liver, skeletal muscle and skin using Anti-MCCC2 antibody NBP2-38403 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: MCCC2 Antibody [NBP2-38403] - Staining of human skin.
Immunohistochemistry-Paraffin: MCCC2 Antibody [NBP2-38403] - Staining of human liver.
Immunohistochemistry-Paraffin: MCCC2 Antibody [NBP2-38403] - Staining of human kidney using Anti-MCCC2 antibody NBP2-38403.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MCCC2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GIAKDGAKMVAAVACAQVPKITLIIGGSYGAGNYGMCGRAYSPRFLYIWPNARISVMGGEQAANVLATITKDQRAREGKQFSSADE
Predicted Species
Mouse (90%), Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCCC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MCCC2 Protein (NBP2-38403PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MCCC2 Antibody

  • 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta
  • MCCase subunit beta
  • MCCBEC 6.4.1.4
  • methylcrotonoyl-CoA carboxylase 2 (beta)
  • methylcrotonoyl-Coenzyme A carboxylase 2 (beta)
  • mitochondrial
  • non-biotin containing subunit of 3-methylcrotonyl-CoA carboxylase

Background

MCCC2 encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MCCC2
Uniprot