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MCCC2 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: MCCC2 Antibody [NBP1-82794] - Staining in human kidney and skeletal muscle tissues using anti-MCCC2 antibody. Corresponding MCCC2 RNA-seq data are presented ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: MCCC2 Antibody [NBP1-82794] - Staining of human kidney, liver, skeletal muscle and skin using Anti-MCCC2 antibody NBP1-82794 (A) shows similar protein ...read more
Independent Antibodies: Western Blot: MCCC2 Antibody [NBP1-82794] - Analysis using Anti-MCCC2 antibody NBP1-82794 (A) shows similar pattern to independent antibody NBP2-38930 (B).
Immunohistochemistry-Paraffin: MCCC2 Antibody [NBP1-82794] - Staining of human skeletal muscle using Anti-MCCC2 antibody NBP1-82794.
Immunohistochemistry-Paraffin: MCCC2 Antibody [NBP1-82794] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: MCCC2 Antibody [NBP1-82794] - Staining of human liver.
Immunohistochemistry-Paraffin: MCCC2 Antibody [NBP1-82794] - Staining of human skin.
Immunohistochemistry-Paraffin: MCCC2 Antibody [NBP1-82794] - Staining of human kidney using Anti-MCCC2 antibody NBP1-82794.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MCCC2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LHISRGKLLPRERIDNLIDPGSPFLELSQFAGYQLYDNEEVPGGGIITGIGRVSGVECMIIANDATVKGGAYYPVTV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCCC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
MCCC2 Protein (NBP1-82794PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MCCC2 Antibody

  • 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta
  • MCCase subunit beta
  • MCCBEC 6.4.1.4
  • methylcrotonoyl-CoA carboxylase 2 (beta)
  • methylcrotonoyl-Coenzyme A carboxylase 2 (beta)
  • mitochondrial
  • non-biotin containing subunit of 3-methylcrotonyl-CoA carboxylase

Background

MCCC2 encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol MCCC2