![Western Blot: MCC Antibody (1A7) [H00004163-M01] - Analysis of MCC expression in transfected 293T cell line by MCC monoclonal antibody (M01), clone 1A7.Lane 1: MCC transfected lysate(93.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MCC-Antibody-1A7-Western-Blot-H00004163-M01-img0006.jpg "Western Blot: MCC Antibody (1A7) [H00004163-M01] - Analysis of MCC expression in transfected 293T cell line by MCC monoclonal antibody (M01), clone 1A7.Lane 1: MCC transfected lysate(93.1 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, KD |
| Clone | 1A7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | MCC (AAH09279, 31 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DIVELNKRLQQTERERDLLEKKLAKAQCEQSHLMREHEDVQERTTLRYEERITELHSVIAELNKKIDRLQGTTIREEDEYSELRSELSQSQHEVNEDSRS |
| Specificity | MCC - mutated in colorectal cancers |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MCC |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MCC Antibody (H00004163-M01)Find related products by research area.
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![Western Blot: MCC Antibody (1A7) [H00004163-M01] - Analysis of MCC over-expressed 293 cell line, cotransfected with MCC Validated Chimera RNAi ( Cat # H00004163-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MCC monoclonal antibody (M01), clone 1A7 (Cat # H00004163-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/MCC-Antibody-1A7-Western-Blot-H00004163-M01-img0004.jpg "Western Blot: MCC Antibody (1A7) [H00004163-M01] - Analysis of MCC over-expressed 293 cell line, cotransfected with MCC Validated Chimera RNAi ( Cat # H00004163-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MCC monoclonal antibody (M01), clone 1A7 (Cat # H00004163-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Sandwich ELISA: MCC Antibody (1A7) [H00004163-M01] - Detection limit for recombinant GST tagged MCC is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MCC-Antibody-1A7-Sandwich-ELISA-H00004163-M01-img0005.jpg "Sandwich ELISA: MCC Antibody (1A7) [H00004163-M01] - Detection limit for recombinant GST tagged MCC is approximately 0.03ng/ml as a capture antibody.")
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