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MAP3K8/Tpl2/COT Antibody (OTI3D9) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
OTI3D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700

Order Details

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MAP3K8/Tpl2/COT Antibody (OTI3D9) [Alexa Fluor® 700] Summary

Immunogen
Recombinant protein fragment corresponding to amino acids 277-461 of human MAP3K8/Tpl2/COT (NP_005195) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAP3K8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MAP3K8/Tpl2/COT Antibody (OTI3D9) [Alexa Fluor® 700]

  • Cancer Osaka thyroid oncogene
  • c-COT
  • cot (cancer Osaka thyroid) oncogene
  • COT
  • COTMEKK8
  • EC 2.7.11.25
  • EST
  • ESTF
  • ESTFLJ10486
  • Ewing sarcoma transformant
  • MAP3K8
  • mitogen-activated protein kinase kinase kinase 8
  • Proto-oncogene c-Cot
  • proto-oncogene serine/threoine protein kinase
  • Serine/threonine-protein kinase cot
  • TPL2
  • Tpl-2
  • Tumor progression locus 2
  • tumor progression locus-2

Background

The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1. To explain these contrasting findings, it has been suggested that Cot, Ras and Raf-1 may form a multimeric complex that phosphorylates MEK-1. Cot has also been shown to be implicated in T lymphocyte activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MAP3K8