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Recombinant Rat MANF Protein

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Concentration
LYOPH

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Recombinant Rat MANF Protein Summary

Description
A single non-glycosylated polypeptide chain containing 158 amino acids corresponding to MANF Source: E. coli

Uniprot ID: P0C5H9

Amino Acid Sequence: LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIEEELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGEMCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
Details of Functionality
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 10 ug/ml, corresponding to a specific activity of > 100 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
MANF
Purity
>98%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of MANF as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
18.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
>98%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Rat MANF Protein

  • Arginine-Rich Protein
  • arginine-rich, mutated in early stage tumors
  • ARMET
  • ARP
  • MANF
  • mesencephalic astrocyte-derived neurotrophic factor
  • Protein ARMET

Background

MANF is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of a specific mutation changing the previously numbered codon 50 from ATG to AGG, or deletion of that codon, has been reported in a variety of solid tumors. With the protein size correction, this codon is now identified as the initiation codon. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Rat
MANF Protein
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Recombinant Rat MANF Protein
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Applications: Bioactivity

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Bioinformatics

Gene Symbol MANF